In this present study we investigated the effects of a saponin fraction prepared from the roots of Panax notoginseng (Burt.) F. H. Chen (Family Araliaceae), on human sperm motility and progression in vitro. Fifteeen human semen samples were collected and qualified according to the criteria of W.H.O. The saponin fraction was evaluated at 1.0 mg/ml and 2.0 mg/ml respectively. The results demonstrate that the fraction increased motility as well as progression of sperm at the 60(th) minute or the 120(th) minute.