Two triterpene saponins (IPS-1, IPS-2) for the first time were isolated from the roots of Impatiens parviflora DC. (Balsaminaceae). Their anti-inflammatory activity was evaluated by means of two in vitro models: anti-hyaluronidase and anti-denaturation assays. Both saponins were shown to be potent hyaluronidase inhibitors that affect the enzyme in a dose-dependent manner. The anti-hyaluronidase effect of IPS-2 (IC50 = 286.7 µg/mL) was higher than that of the reference drug: escin (IC50 = 303.93 µg/mL). Both saponins protected bovine serum albumin from heat-induced denaturation in a dose-dependent manner. IPS-1 demonstrated higher anti-denaturation effect (IC50 = 86.7 µg/ml) than IPS-2 (IC50 = 109.76 µg/mL) or the standard drug: acetylsalicylic acid (IC50 = 262.22 µg/mL). In conclusion, potent activity of IPS-1, IPS-2 in both in vitro assays shows that saponins from I. parviflora have anti-inflammatory activity. The obtained results allow to suggest that such compounds may be beneficial in inflammatory conditions, especially associated with excessive degradation of hyaluronic acid.

译文

:首次从小凤仙花DC的根中分离出两个三萜皂苷(IPS-1,IPS-2)。 (鼠尾草科)。通过两种体外模型评估它们的抗炎活性:抗透明质酸酶和抗变性测定。两种皂苷均被证明是有效的透明质酸酶抑制剂,以剂量依赖的方式影响该酶。 IPS-2(IC50- = 286.7ICµg / mL)的抗透明质酸酶作用高于参考药物七叶皂苷(IC50 = 303.93 µg / mL)。两种皂苷均以剂量依赖性方式保护牛血清白蛋白免于热诱导的变性。 IPS-1具有比IPS-2(IC50 = 109.76µg / mL)或标准药物乙酰水杨酸(IC50 = 262.22µg / mL)更高的抗变性作用(IC50 = 86.7(µg / mL)。总之,在两种体外测定中,IPS-1,IPS-2的有效活性均显示小花伊豆中的皂苷具有抗炎活性。所获得的结果表明,这类化合物在炎性疾病中可能是有益的,特别是与透明质酸的过度降解有关。

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