Rotavirus is one of the leading causes of acute gastroenteritis in infants and young children. RVAs infect not only humans but also a wide range of mammals including rats, which represent a reservoir of several other zoonotic pathogens. Due to the segmented nature of the RVA genome, animal RVA strains can easily adapt to the human host by reassortment with co-infecting human viruses. This study aims to detect and characterize RVA in the intestinal content of Italian sinantropic rats (Rattus rattus). Out of 40 samples examined following molecular approach, one resulted positive for RVA. The molecular characterization of VP1-4, 6 and 7, and NSP1-5 genes by sequencing revealed the genomic constellation G3-P[3]-I1-R11-C11-M10-A22-N18-T14-E18-H13. This uncommon genomic combination includes: the VP1-4,VP7, the NSP1, 3, 4 and 5 gene segments, closely related to those of RVA from rodents, the N18 novel genotype established for the NSP2 gene segment and the human Wa-like VP6 gene, suggesting interspecies reassortment.

译文

:轮状病毒是婴幼儿急性胃肠炎的主要原因之一。 RVA不仅感染人类,还感染包括鼠在内的多种哺乳动物,它们代表着几种其他人畜共患病原体。由于RVA基因组的分段性质,动物RVA株可通过与共同感染人类病毒的重配轻松地适应人类宿主。这项研究旨在检测和表征意大利鼻中风大鼠(Rattus rattus)肠内的RVA。采用分子方法检查的40个样品中,有一个RVA呈阳性。通过测序对VP1-4、6和7和NSP1-5基因进行分子表征,揭示了基因组构象G3-P [3] -I1-R11-C11-M10-A22-N18-T14-E18-H13。这种罕见的基因组组合包括:VP1-4,VP7,NSP1、3、4和5基因片段,与啮齿动物的RVA紧密相关,为NSP2基因片段建立的N18新基因型和人类Wa样VP6基因,提示种间重配。

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