OBJECTIVE:The aim of the present investigation was to characterize the effects of supraphysiological doses of the anabolic androgenic steroid nandrolone decanoate (ND) on the fertility of female rats, as well as on the morphology of their uterus. STUDY DESIGN:Female Wistar rats (n=15) received a subcutaneous injection of ND (15 mg/kg) once daily during a 2-week period, while the control animals (n=10) were administered vehicle alone (arachidis oleum) in the same manner. Estrus behavior was evaluated 4 weeks after termination of this treatment and in cases where signs of receptivity were present, the female rat was given the opportunity to copulate with a male. After breeding, the female animals were sacrificed and their uteri examined histomorphologically. RESULTS:All ND-treated animals exhibited abnormal vaginal smears, whereas all of the control smears were normal. Most (73%) of the treated females demonstrated normal estrus behavior (i.e., willingness) on the day of mating, but none got pregnant; whereas all of the control rats became pregnant. The female rats receiving the ND showed an enhanced rate of weight gain and the myometrium thickness of their uteri was significantly increased, while the endometrium was significantly thinner. Furthermore, ND caused a significant proportion of the treated animals to display tortuous and irregularly branching endometrial glands, as well as a lack of the physiologically normal infiltration of eosinophilic leukocytes into the endometrium (endometrial eosinophilic homing), a finding that has not been reported previously. CONCLUSION:The present findings indicate that high doses of ND cause morphological and physiological alterations in the uterus of female rats that are associated with a suppression of their reproductive capacity.

译文

目的:本研究的目的是表征同化雄性类固醇癸酸nandrolone癸酸酯的超生理剂量对雌性大鼠生育能力以及子宫形态的影响。
研究设计:Wistar雌性大鼠(n = 15)在2周内每天一次皮下注射ND(15 mg / kg),而对照组(n = 10)单独给予媒介物(花生油)。同样的方式。终止该治疗4周后评估发情行为,并且在出现接受迹象的情况下,给予雌性大鼠与雄性交配的机会。繁殖后,将雌性动物处死并对其子宫进行组织形态学检查。
结果:所有接受ND治疗的动物均表现出异常的阴道涂片,而所有对照涂片均正常。接受治疗的大多数女性(73%)在交配当天表现出正常的发情行为(即意愿),但没有人怀孕;而所有对照大鼠都怀孕了。接受ND的雌性大鼠体重增加率增加,子宫肌层厚度明显增加,而子宫内膜明显变薄。此外,ND导致相当一部分被治疗的动物表现出曲折和不规则分支的子宫内膜腺体,并且缺乏正常的嗜酸性白细胞进入子宫内膜的生理正常浸润(子宫内膜嗜酸性归巢),这一发现以前未见报道。 。
结论:本研究结果表明,高剂量的ND可导致雌性大鼠子宫形态和生理改变,从而抑制其生殖能力。

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