UNLABELLED:In this study, protective effects of adenosine and acetylcholine-induced postconditioning were investigated on the contractile function of the ischemic isolated rat ventricular myocytes. A video-based edge-detection system was used to monitor single ventricular myocytes contraction. Adenosine and acetylcholine were administrated for 6 min before ischemia as preconditioning, or 15 min after ischemia as postconditioning. Adenosine and acetylcholine receptor antagonists and mitoKATP inhibitor were used to analyze pathways underlying the effects on postconditioning. RESULTS:(1) The peak shortening of ischemic heart cells was improved by both adenosine and acetylcholine during preconditioning (84.72+/-5.34% and 68.61+/-8.10% vs. control: 8.43+/-5.35% of the pre-ischemia value), as well as postconditioning (76.47+/-7.87% and 57.48+/-6.97% vs. control: 8.43+/-5.35% of the pre-ischemia value) and the effects of preconditioning and postconditioning were comparable. More datum in the normal text. (2) Observed effects of adenosine and acetylcholine postconditioning were missing in the presence of adenosine A1 receptor and muscarinic M2 receptor antagonists, respectively. (3) Adenosine and acetylcholine-induced postconditioning was also blocked by mitoKATP antagonist. These results suggest that both adenosine and acetylcholine protect the contractile function of ischemic heart cells to a similar extent during preconditioning and postconditioning. The postconditioning of adenosine and acetylcholine is relative to the adenosine A1 and muscarinic M2 receptors, respectively. MitoKATP is implicated in the postconditioning of both acetylcholine and adenosine.

译文

未标记:在本研究中,研究了腺苷和乙酰胆碱诱导的后处理对缺血性离体大鼠心室肌细胞收缩功能的保护作用。基于视频的边缘检测系统用于监测单个心室肌细胞的收缩。缺血前6分钟给予腺苷和乙酰胆碱作为预处理,缺血后15分钟给予腺苷和乙酰胆碱作为后处理。腺苷和乙酰胆碱受体拮抗剂以及mitoKATP抑制剂被用于分析对后处理的影响的潜在途径。
结果:(1)腺苷和乙酰胆碱在预处理过程中均改善了缺血性心脏细胞的峰缩短(与对照组相比分别为84.72 /-5.34%和68.61 /-8.10%:缺血前值的8.43 /-5.35%),以及后处理(相对于对照组,分别为76.47 /-7.87%和57.48 /-6.97%:缺血前值的8.43 /-5.35%),并且预处理和后处理的效果可比。普通文本中的基准面更多。 (2)在腺苷A1受体和毒蕈碱M2受体拮抗剂的存在下,腺苷和乙酰胆碱后处理的观察到的作用分别消失了。 (3)腺苷和乙酰胆碱引起的后处理也被mitoKATP拮抗剂阻断。这些结果表明,腺苷和乙酰胆碱在预处理和后处理过程中均以相似的程度保护缺血性心脏细胞的收缩功能。腺苷和乙酰胆碱的后处理分别相对于腺苷A1和毒蕈碱M2受体。 MitoKATP与乙酰胆碱和腺苷的后处理有关。

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