The gene encoding a single-chain, ribosome-inactivating protein (SCRIP) was cloned from bitter melon (Momordica charantia L.) leaves infected with the fungus, Sphaerotheca fuliginea, by RT-PCR. The ORF was 861 bp. The ribosome-inactivating protein was expressed in E. coli and, when purified, it inhibited the growth of Fusarium solani [corrected] Northern blot analysis revealed that RIP transcripts rapidly accumulated in leaves 1-day post inoculation with Sphaerotheca fuliginea and reached a peak at 3 d. The expression pattern of RIP induced by methyl jasmonate and salicylic acid were different from that of pathogen-induced expression. Mechanical wounding, silver nitrate and osmotic stress stimulated only a slight accumulation of RIP transcripts. Abscisic acid also induced transcription of RIPs. The signal compounds, ethylene and okadaic acid, induced a moderate accumulation of RIP transcripts.

译文

:通过RT-PCR从感染了真菌Sphaerotheca fuliginea的苦瓜(Momordica charantia L.)叶片中克隆了编码单链核糖体失活蛋白(SCRIP)的基因。 ORF为861bp。核糖体失活蛋白在大肠杆菌中表达,纯化后抑制了茄镰刀菌的生长。 3天茉莉酸甲酯和水杨酸诱导的RIP表达模式与病原体诱导的表达模式不同。机械伤,硝酸银和渗透胁迫仅刺激了RIP转录物的少量积累。脱落酸还诱导RIP的转录。信号化合物,乙烯和冈田酸,诱导了RIP转录产物的适度积累。

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