Detergent-resistant membranes (DRMs) represent specialized membrane domains resistant to detergent extraction, which may serve to segregate proteins in a specific environment in order to improve their function. Segregation of glycosylphosphatidylinositol-anchored proteins (GPI-APs) in DRMs has been shown to be involved in their sorting to the apical membrane in polarized epithelial cells. Nonetheless, we have shown that both apical and basolateral GPI-APs associate with DRMs. In this report we investigated the lipid composition of DRMs associated with an apical and a basolateral GPI-AP. We found that apical and basolateral DRMs contain the same lipid species although in different ratios. This specific lipid ratio is maintained after mixing the cells before lysis indicating that DRMs maintain their identity after Triton extraction.

译文

:抗洗涤剂膜(DRMs)代表对去污剂萃取有抗性的特殊膜结构域,可用于在特定环境中分离蛋白质以改善其功能。研究表明,DRM中糖基磷脂酰肌醇锚定蛋白(GPI-AP)的分离与其在极化上皮细胞的顶膜中的分选有关。尽管如此,我们已经表明,根尖和基底外侧的GPI-AP均与DRM相关。在本报告中,我们研究了与根尖和基底外侧GPI-AP相关的DRM的脂质成分。我们发现顶端和基底外侧DRM包含相同的脂质种类,尽管比率不同。在裂解之前混合细胞后,该特定脂质比率得以维持,这表明DRM在Triton提取后仍保持其身份。

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