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A high level of drug resistance was obtained with a line of Plasmodium chabaudi maintained under intense chloroquine selection pressure according to the protocols established for P. berghei. The main objective of this work was to verify if the characteristic asynchronous schizogonic rhythm of naturally resistant rodent malaria parasites was also found when the drug resistance was induced experimentally. The degree of resistance was evaluated through the use of the "2% delay test" (DT) and the schizogonic rhythm, by reference to the synchronicity index (SI). The strain had originally a DT of 4.26 and an SI of 0.52. Following the application of 80 mg/kg chloroquine at each passage, as early as at the 8th passage the parasites rapidly became resistant and asynchronous. At the 17th passage the DT was 3.32 and the SI, 0.20. In the drug-resistant line the original indices, both the DT and the SI, were restored after deep-freezing, sporogony, or passage through a Percoll gradient, or simply by repeated intravenous subinoculations of blood. The clear correlation between asynchronicity and drug resistance is easily explained by the action of chloroquine, which favours the schizogonic cycles initiated by latent merozoites.

译文

根据针对伯氏疟原虫建立的操作规程,在强氯喹选择压力下维持的夏巴疟原虫系列获得了高水平的耐药性。这项工作的主要目的是验证通过实验诱导耐药性时是否还发现了自然抗性啮齿动物疟疾寄生虫的特征性异步精神分裂节律。通过使用“ 2%延迟测试”(DT)和精神分裂节律,并参考同步指数(SI)评估抵抗程度。该菌株最初的DT为4.26,SI为0.52。在每次传代施用80 mg / kg氯喹后,早在第8次传代时,寄生虫便迅速变得具有抗性和异步性。在第17遍时,DT为3.32,SI为0.20。在抗药性品系中,深层冷冻,孢子形成或通过Percoll梯度或简单地通过重复静脉内亚接种血后,即恢复了DT和SI的原始指标。氯喹的作用很容易解释异步性和耐药性之间的明确关系,这有利于潜伏裂殖子引发的精神分裂循环。

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