OBJECTIVE:To examine the relationship between infection with Mycoplasma and the development of rheumatoid arthritis (RA) and juvenile rheumatoid arthritis (JRA).

METHODS:Immunoblotting of patient synovial fluid and sera on detergent-phase membrane protein extracts of various Mycoplasma species was carried out to learn whether patients exhibited serologic evidence of previous exposure to mycoplasmas. Moreover, an ultrasensitive polymerase chain reaction (PCR) method was developed for assessing whether Mycoplasma DNA could be detected in synovial fluid from patients and controls.

RESULTS:Immunoblotting provided serologic evidence of previous Mycoplasma exposure in patients and controls. The genus-specific PCR detected known human Mycoplasma species and could reliably detect <5 copies of Mycoplasma hominis, Mycoplasma fermentans, or a molecular mimic control in synovial fluid. Repeat testing revealed no evidence of Mycoplasma DNA in patient synovial samples.

CONCLUSION:This study provided serologic evidence suggesting that, while previous exposure to Mycoplasma was common, there was no detectable persistence of Mycoplasma DNA in the synovial fluid or tissue of patients with RA or JRA.

译文

目标:探讨支原体感染与类风湿关节炎(RA)和青少年类风湿关节炎(JRA)的发展之间的关系。

方法:免疫印迹通过对各种支原体物种的去污剂相膜蛋白提取物的滑膜液和血清进行分析,以了解患者是否表现出先前接触过支原体的血清学证据。此外,还开发了一种超灵敏的聚合酶链反应(PCR)方法,用于评估是否可以从患者和对照的滑液中检测到支原体DNA。

结果:免疫印迹提供了先前的血清学证据患者和对照中的支原体暴露。属特异性PCR检测到已知的人类支原体物种,并且可以可靠地检测到<5个拷贝的人支原体,发酵支原体或滑液中的分子模拟对照。重复测试未发现患者滑膜样品中有支原体DNA的证据。

结论:该研究提供了血清学证据,表明尽管先前接触过支原体是很普遍的,但没有发现可持久存在的支原体。 RA或JRA患者滑液或组织中的支原体DNA。

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