Mycobacterial interspersed repetitive units variable number tandem repeat (MIRU-VNTR) typing of Mycobacterium tuberculosis complex (MTBC) isolates, based on 24 loci, is still widely used as the standard for routine molecular surveillance of tuberculosis (TB). QIAxcel system is proposed as an affordable tool that could replace conventional gel electrophoresis and provide high concordance with the reference methods regarding MIRU-VNTR typing of MTBC. We aimed to evaluate the QIAxcel accuracy for allele calling of MIRU-VNTR loci in two regional reference laboratories. A total of 173 DNA were used for the study. Results obtained with QIAxcel were compared to the reference results obtained with an ABI 3730 DNA analyzer. In Albania, the overall agreement with the reference method was 97.92%. A complete agreement result was obtained for 17 loci. In Tunisia, the overall agreement with the reference method was 98.95%. A complete agreement result was obtained for 17 loci. Overall agreement in both centers was 98.43%. In our opinion, use of QIAxcel technology has the potential to be reliable, given an optimized algorithm. Inaccuracies in sizing of long fragments should be solved, especially regarding locus 4052.

译文

结核分枝杆菌复合物(MTBC)分离株的分枝杆菌重复单元可变数目串联重复序列(MIRU-VNTR)分型,基于24个基因座,仍广泛用作常规的结核病分子监测(TB)标准。 QIAxcel系统被认为是一种经济实惠的工具,可以代替传统的凝胶电泳,并与有关MTBC的MIRU-VNTR分型的参考方法高度一致。我们旨在评估两个区域参考实验室中MIRU-VNTR基因座等位基因调用的QIAxcel准确性。该研究总共使用了173个DNA。将使用QIAxcel获得的结果与使用ABI 3730 DNA分析仪获得的参考结果进行比较。在阿尔巴尼亚,与参考方法的总体一致性为97.92%。获得了17个基因座的完整一致性结果。在突尼斯,与参考方法的总体一致性为98.95%。获得了17个基因座的完整一致性结果。两个中心的总体协议率为98.43%。我们认为,在优化算法的情况下,使用QIAxcel技术具有可靠的潜力。应该解决长片段大小不正确的问题,尤其是在4052位点上。

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