To achieve high time-space efficiency for sophorolipid production with yeast Candida bombicola, a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal cell density and (2) the computer-aided adjustment of physical parameters and the controlled feeding of substrates for enhanced volumetric productivity. Both stages have been successfully implemented in a 10-L fermenter, where up to 80 g dry cell weight/L was obtained and a remarkably high volumetric productivity (> 200 g isolated sophorolipids/L/day) was achieved. Both the biomass and volumetric productivity were markedly higher than previously reported. Specifically, the high productivity of sophorolipids could be attained on a very short time scale (24 h), highlighting the industrial potential of the platform developed in this work.

译文

为了实现酵母念珠菌生产槐糖脂的高时空效率,采用了高细胞密度发酵策略。该方法包括两个连续的阶段 :( 1) 优化碳源和养分浓度以实现最大细胞密度; (2) 计算机辅助调节物理参数和受控进料底物以提高体积生产率。这两个阶段都已在10-l发酵罐中成功实施,其中获得了高达80 g的干细胞重量/L,并且实现了非常高的体积生产率 (> 200g分离的槐糖脂/L/天)。生物量和体积生产率均明显高于先前报道的水平。具体来说,可以在非常短的时间范围内 (24小时) 实现槐糖脂的高生产率,这突显了在这项工作中开发的平台的工业潜力。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录