Catch crop candidates (corn, guinea grass) for recovering nutrients from farm soil and aquatic plants (water caltrop, water hyacinth) were utilized to produce L-lactic acid. The efficiencies ofpre-treatment methods for enzymatic saccharification and L-lactate production of two fermentation processes, thermophilic simultaneous saccharification and fermentation (SSF), as well as separate saccharification and fermentation, were compared. Conditions were set at 55 degrees C and pH 5.5 for non-sterile fermentation. Alkaline/peroxide pre-treatment proved the most effective for saccharification in pre-treated corn, guinea grass, water caltrop and water hyacinth with glucose yields of 0.23, 0.20, 0.11 and 0.14 g/g-dry native biomass (24-hour incubation period), respectively. Examination of the two types of thermophilic L-lactate fermentation employed following alkaline/peroxide pre-treatment and saccharification demonstrated that the L-lactate yield obtained using SSF (0.15 g/g in the case of corn) was lower than that obtained using separate saccharification and fermentation (0.28 g/g in the case of corn). The lower yield obtained from SSF is likely to have resulted from the saccharification conditions used in the present study, as the possibility of cellulase deactivation during SSF by thermophilic L-lactate producing bacteria existed. A cellulase that retains high activity levels under non-sterile conditions and a L-lactate producer without cellulose hydrolysis activity would be required in order for SSF to serve as an effective method of L-lactate production.

译文

用于从农场土壤和水生植物 (水caltrop,水葫芦) 中回收养分的农作物候选物 (玉米,几内亚草) 用于生产L-乳酸。比较了嗜热同时糖化和发酵 (SSF) 以及单独糖化和发酵两种发酵过程的酶促糖化和L-乳酸生产预处理方法的效率。将非无菌发酵的条件设定在55 ℃ 和pH 5.5。碱性/过氧化物预处理被证明对经预处理的玉米,豚草,水caltrop和水葫芦的糖化最有效,葡萄糖产量分别为0.23,0.20,0.11和0.14g/g干天然生物质 (24小时孵育期)。对碱性/过氧化物预处理和糖化后使用的两种嗜热L-乳酸发酵的检查表明,使用SSF获得的L-乳酸产量 (玉米为0.15g/g) 低于使用单独糖化和发酵获得的L-乳酸产量 (在该情况下为0.28g/g) 玉米)。从SSF获得的较低产量可能是由于本研究中使用的糖化条件所致,因为存在嗜热L-乳酸产生细菌在SSF期间纤维素酶失活的可能性。为了使SSF用作生产L-乳酸的有效方法,将需要在非无菌条件下保持高活性水平的纤维素酶和无纤维素水解活性的L-乳酸生产者。

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