Although Alpinia officinarum has been used in traditional medicine for the treatment of several conditions, such as abdominal pain, emesis, diarrhea, impaired renal function, and dysentery, little is known about its function in obesity. In this study, we investigated the antiobesity effect of A. officinarum ethanol extract (AOE) on lipid accumulation in 3T3-L1 cells and obesity in mice fed a high-fat diet (HFD). AOE dose-dependently suppressed lipid accumulation during differentiation of 3T3-L1 preadipocytes by downregulating CCAAT enhancer binding protein α (C/EBPα), sterol regulatory element binding protein-1 (SREBP-1), and peroxisome proliferator-activated receptor-γ (PPAR-γ) genes. Galangin, a major component of A. officinarum, had antiadipogenic effects in 3T3-L1 cells. AOE supplementation in mice fed a HFD revealed that AOE significantly decreased HFD-induced increases in body, liver, and white adipose tissue weights and decreased serum insulin and leptin levels. To elucidate the inhibitory mechanism of AOE in obesity, lipid metabolism-related genes were identified. AOE efficiently suppressed protein expressions of C/EBPα, fatty acid synthase, SREBP-1, and PPAR-γ in the liver and adipose tissue. The protein expression patterns, observed by immunoblot, were confirmed by quantitative real-time polymerase chain reaction. Collectively, these results suggest that AOE prevents obesity by suppressing adipogenic and lipogenic genes. AOE has potential for use as an antiobesity therapeutic agent that can function by regulating lipid metabolism.

译文

尽管高山姜已在传统医学中用于治疗多种疾病,例如腹痛,呕吐,腹泻,肾功能受损和痢疾,但对其在肥胖症中的功能知之甚少。在这项研究中,我们研究了A. officinarum乙醇提取物 (AOE) 对高脂饮食 (HFD) 小鼠3T3-L1细胞脂质积累和肥胖的抗肥胖作用。AOE通过下调CCAAT增强子结合蛋白 α (C/ebp α),固醇调节元件结合蛋白1 (SREBP-1) 和过氧化物酶体增殖物激活受体-γ (PPAR-γ) 基因,剂量依赖性地抑制3T3-L1前脂肪细胞分化过程中的脂质积累。高良姜是a.officinarum的主要成分,在3T3-L1细胞中具有抗脂肪作用。在喂食HFD的小鼠中补充AOE表明,AOE显着降低了HFD诱导的身体,肝脏和白色脂肪组织重量的增加,并降低了血清胰岛素和瘦素水平。为了阐明AOE对肥胖的抑制机制,确定了脂质代谢相关基因。AOE可有效抑制肝脏和脂肪组织中C/ebp α,脂肪酸合酶,SREBP-1和PPAR-γ 的蛋白表达。通过定量实时聚合酶链反应证实了通过免疫印迹观察到的蛋白质表达模式。总的来说,这些结果表明AOE通过抑制成脂和生脂基因来预防肥胖。AOE具有用作抗肥胖治疗剂的潜力,可以通过调节脂质代谢来发挥作用。

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