Four strains of ascomycetous yeasts were isolated from samples collected at two locations in southern Japan. The strains formed two warty ascospores that were joined together by an intersporal body appearing as a belt. Phylogenetic analysis of rRNA gene nucleotide sequences indicated that the strains represented two new and closely related species of the genus Kazachstania. Isolates of one of the species were from Miyazaki Prefecture and those of the other species were from the Iriomote Islands. Genetic separation of the two species was further confirmed by DNA-DNA reassociation, which gave values of 63.3-78.1%, and from interspecific crosses, which gave nonviable ascospores. On the basis of these data, the isolates from Miyazaki Prefecture are described as Kazachstania zonata sp. nov. [type strain NBRC 100504=CBS 10326, mating types NBRC 101821 (+), NBRC 101822 (-)], and the isolates from the Iriomote Islands are described as Kazachstania gamospora sp. nov. [type strain NBRC 11056=CBS 10328, mating types NBRC 101825 (+), NBRC 101826 (-)].
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【Two new species of Kazachstania that form ascospores connected by a belt-like intersporal body: Kazachstania zonata and Kazachstania gamospora.].
【由带状的孢子间体连接形成子囊孢子的两个新种: Kazachstania zonata和Kazachstania gamospora。】
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DOI：10.1111/j.1567-1364.2006.00163.x文章类型：杂志文章
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从日本南部两个地点收集的样品中分离出四株子囊菌酵母。菌株形成了两个疣状子囊孢子，这些子囊孢子通过出现为带的孢子间体连接在一起。rRNA基因核苷酸序列的系统发育分析表明，该菌株代表了Kazachstania属的两个新的且密切相关的物种。其中一个物种的分离株来自宫崎县，其他物种的分离株来自西表群岛。通过DNA-DNA重新结合进一步证实了这两个物种的遗传分离，这给出了63.3-78.1% 的值，而种间杂交则给出了不可存活的子囊孢子。根据这些数据，来自宫崎县的分离株被描述为Kazachstania zonata sp。11月[类型菌株NBRC 100504 = CBS 10326，交配类型NBRC 101821 ()，NBRC 101822 (-)] 和来自西表岛的分离株被描述为Kazachstania gamospora sp。11月[类型菌株NBRC 11056 = CBS 10328，配对类型NBRC 101825 (+)，NBRC 101826 (-)]。
