Expression of genes coding for ubiquitin and heatshock protein (hsp) 70 were examined by in situ hybridization using a rat model with permanent occlusion of the distal middle cerebral artery (MCA). Only polyubiquitin (UbC) mRNA increased markedly following ischaemia in the central zone of the MCA territory of the neocortex. UbC gene expression reached the maximum level 4 h post-occlusion and remained elevated at 24 h. UbC expression was retarded slightly compared with that of the hsp70 gene. UbB and Ub-S30 were expressed at almost similar levels in both the ischaemic and non-ischaemic hemispheres. These results indicated that UbC probably has the most stress-inducible characteristics among the three ubiquitin genes.

译文

使用具有远端大脑中动脉 (MCA) 永久闭塞的大鼠模型,通过原位杂交检查了编码泛素和热休克蛋白 (hsp) 70的基因的表达。在新大脑皮层MCA区域的中央区域发生缺血后,只有聚泛素 (UbC) mRNA显着增加。UbC基因表达在闭塞后4小时达到最大水平,并在24小时保持升高。与hsp70基因相比,UbC表达略有延迟。在缺血性和非缺血性半球中,UbB和Ub-S30的表达水平几乎相似。这些结果表明,在三个泛素基因中,UbC可能具有最易受胁迫诱导的特征。

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