The hazards of using optic nerve (as opposed to optic tract and more distal components of the optic system) to study axonal transport were highlighted by observing the fate of [14C]serine and [3H]glycerol injected into the rabbit eye. Despite prior blockage of axonal transport with colchicine, appreciable radioactivity rapidly appeared in the optic nerve adjacent to the injected eye. Radioactivity decreased exponentially along the entire optic chiasm. Counts were distributed among the lipid, protein, and acid-soluble fractions. Separation of optic nerve lipids revealed appreciable labeling of most lipid classes including those characteristic of myelin; a markedly different labeling pattern was observed for axonally transported lipids. The data are consistent with a mechanism involving extra-axonal diffusion of precursor into the surrounding glia followed by incorporation into lipids and proteins of those cells and ultimately myelin. The phenomenon is discussed in relation to possible errors that were made in interpreting earlier experiments.

译文

通过观察注射到兔眼中的 [14C] 丝氨酸和 [3H] 甘油的命运,突出了使用视神经 (相对于视神经系统和更多视神经系统的远端成分) 来研究轴突运输的危害。尽管先前用秋水仙碱阻断了轴突运输,明显的放射性迅速出现在与注射的眼睛相邻的视神经中。放射性沿整个视交叉呈指数下降。计数分布在脂质、蛋白质和酸溶性部分中。视神经脂质的分离揭示了大多数脂质类别的明显标记,包括髓磷脂的特征; 对于轴突运输的脂质观察到明显不同的标记模式。数据与涉及前体轴突外扩散到周围神经胶质的机制一致,然后掺入到这些细胞的脂质和蛋白质以及最终髓磷脂中。讨论了与解释早期实验中可能出现的错误有关的现象。

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