Aquaporins (AQPs) facilitate water transport across epithelia and play an important role in normal physiology and disease in the human airways. We used in situ hybridization and immunofluorescence to determine the expression and cellular localization of AQPs 5, 4, and 3 in human airway sections. In nose and bronchial epithelia, AQP5 is expressed at the apical membrane of columnar cells of the superficial epithelium and submucosal gland acinar cells. AQP4 was detected in basolateral membranes in ciliated ducts and by in situ in gland acinar cells. AQP3 is present on basal cells of both superficial epithelium and gland acinus. In these regions AQPs 5, 4, and 3 are appropriately situated to permit transepithelial water permeability. In the small airways (proximal and terminal bronchioles) AQP3 distribution shifts from basal cell to surface expression (i.e., localized to the apical membrane of proximal and terminal bronchioles) and is the only AQP identified in this region of the human lung. The alveolar epithelium has all three AQPs represented, with AQP5 and AQP4 localized to type I pneumocytes and AQP3 to type II cells. This study describes an intricate network of AQP expression that mediates water transport across the human airway epithelium.

译文

水通道蛋白 (AQPs) 促进水跨上皮细胞的运输,并在人类呼吸道的正常生理和疾病中起重要作用。我们使用原位杂交和免疫荧光来确定人气道切片中AQPs 5、4和3的表达和细胞定位。在鼻和支气管上皮中,AQP5在浅表上皮和粘膜下腺泡细胞的柱状细胞的顶膜上表达。在纤毛导管的基底外侧膜中以及腺泡细胞的原位检测到AQP4。AQP3存在于浅表上皮和腺泡的基底细胞上。在这些区域中,AQPs 5、4和3的位置适当,以允许跨上皮透水。在小气道 (近端和末端细支气管) 中,AQP3的分布从基底细胞转移到表面表达 (即,定位于近端和末端细支气管的顶膜),并且是在人类肺的该区域中唯一鉴定的AQP。肺泡上皮具有所有三个aqp代表,其中AQP5和AQP4定位于I型肺细胞,AQP3定位于II型细胞。这项研究描述了一个复杂的AQP表达网络,该网络介导水跨人类气道上皮的运输。

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