Aquaporin 0 (AQP0) and AQP1 are expressed in the lens, each in a different cell type, and their functional roles are not thoroughly understood. Our previous study showed that these two AQPs function as water transporters. In order to further understand the functional significance of these two different aquaporins in the lens, we investigated their initiation and continued expression. AQP0 transcript and protein were first detected at embryonic stage (E) 11.25 in the differentiating primary fiber cells of the developing lens; its synthesis continued through the adult stage in the secondary fiber cells. Low levels of AQP1 expression were first seen in lens anterior epithelial cells at E17.5; following postnatal day (P) 6.5, the expression gradually progressed towards the equatorial epithelial cells. In the postnatal lens, the increase in membrane water permeability of epithelial cells and lens transparency coincides with the increase in AQP1 expression. AQP1 expression reaches its peak at P30 and continues through the adult stage both in the anterior and equatorial epithelial cells. The enhancement in AQP1 expression concomitant with the increase in the size of the lens suggests the progression in the establishment of the lens microcirculatory system. In vitro and in vivo studies show that both aquaporins share at least one important function, which is water transport in the lens microcirculatory system. However, the temporal expression of these two AQPs suggests an apparently unique role/s in lens development and transparency. To our knowledge, this is the first report on the expression patterns of AQP0 and AQP1 during lens development and differentiation and their relation to lens transparency.

译文

水通道蛋白0 (AQP0) 和AQP1在晶状体中表达,每种细胞类型不同,其功能作用尚未完全了解。我们先前的研究表明,这两种aqp充当水转运蛋白。为了进一步了解这两种不同水通道蛋白在晶状体中的功能意义,我们研究了它们的起始和持续表达。首先在胚胎期 (E) 11.25在发育中的晶状体的分化原代纤维细胞中检测到AQP0转录物和蛋白质; 其合成在次级纤维细胞中持续到成年阶段。在E17.5时,首先在晶状体前上皮细胞中观察到低水平的AQP1表达; 出生后第 (P) 6.5天,表达逐渐向赤道上皮细胞发展。在产后晶状体中,上皮细胞的膜水通透性和晶状体透明度的增加与AQP1表达的增加相吻合。AQP1的表达在P30达到峰值,并在前上皮细胞和赤道上皮细胞中持续到成年阶段。AQP1表达的增强伴随着晶状体大小的增加,表明建立晶状体微循环系统的过程。体外和体内研究表明,两种水通道蛋白至少具有一个重要功能,即晶状体微循环系统中的水运输。但是,这两个aqp的时间表达表明在晶状体发育和透明度中显然具有独特的作用。据我们所知,这是有关AQP0和AQP1在晶状体发育和分化过程中的表达模式及其与晶状体透明度的关系的第一份报告。

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