Cyclic diguanylate (c-di-GMP) is a broadly conserved bacterial signalling molecule that modulates diverse cellular processes, such as biofilm formation, colony morphology and swimming motility. The intracellular level of c-di-GMP is controlled by diguanylate cyclases (DGCs) with GGDEF domain and phosphodiesterases (PDEs) with either EAL or HD-GYP domain. Pseudomonas putida KT2440 has a large group of genes on its genome encoding proteins with GGDEF/EAL/HD-GYP domains. However, phenotypic-genotypic correlation and c-di-GMP metabolism of these genes were largely unknown. Herein, by systematically constructing deletion mutants/overexpression strains of the 42 predicted c-di-GMP metabolism-related genes and analysing the phenotypes, we preliminarily revealed the role of each gene in biofilm formation, colony morphology and swimming motility. Subsequent results from protein sequence alignments and cellular c-di-GMP assessment indicated that 25 out of the 42 genes were likely to encode DGCs, nine genes were predicted to encode PDEs, four genes encoded bifunctional enzymes and the other four genes encoded enzymatically inactive proteins. This study offers a basic understanding of the roles of these 42 genes and can serve as a toolkit for investigators to further elucidate the functions of these GGDEF and EAL/HD-GYP domain-containing proteins.

译文

环二鸟苷酸 (c-di-GMP) 是一种广泛保守的细菌信号分子,可调节多种细胞过程,例如生物膜形成,菌落形态和游泳运动。c-di-GMP的细胞内水平由具有GGDEF结构域的双鸟苷酸环化酶 (dgc) 和具有EAL或HD-GYP结构域的磷酸二酯酶 (pde) 控制。恶臭假单胞菌KT2440在其基因组上有大量基因,编码具有GGDEF/EAL/hd-gyp结构域的蛋白质。然而,这些基因的表型-基因型相关性和c-di-GMP代谢在很大程度上是未知的。在此,通过系统构建42个预测的c-di-GMP代谢相关基因的缺失突变体/过表达菌株并分析表型,我们初步揭示了每个基因在生物膜形成,菌落形态和游泳运动中的作用。蛋白质序列比对和细胞c-di-GMP评估的后续结果表明,42个基因中有25个可能编码DGCs,预计有9个基因编码pde,4个基因编码双功能酶,其他4个基因编码酶无活性蛋白。这项研究提供了对这42个基因作用的基本理解,并可以作为研究人员进一步阐明这些GGDEF和EAL/hd-gyp结构域蛋白功能的工具包。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录