Pseudomonas aeruginosa is a highly resistant opportunistic pathogen and an important etiological agent of various types of infections. During the last decade, P. aeruginosa phages have been extensively examined as alternative antimicrobial agents. The aim of the study was to determine antimicrobial effectiveness of combining subinhibitory concentrations of gentamicin, ceftriaxone, ciprofloxacin or polymyxin B with P. aeruginosa-specific bacteriophages belonging to families Podoviridae and Siphoviridae. The time-kill curve method showed that a combination of bacteriophages and subinhibitory concentrations of ceftriaxone generally reduced bacterial growth, and synergism was proven for a Siphoviridae phage σ-1 after 300 min of incubation. The detected alteration in morphology after ceftriaxone application, resulting in cell elongation, along with its specific mode of action, seemed to be a necessary but was not a sufficient reason for phage-antibiotic synergism. The phenomenon offers an opportunity for future development of treatment strategies for potentially lethal infections caused by P. aeruginosa.

译文

铜绿假单胞菌是一种高度耐药的机会致病菌,是各种类型感染的重要病原体。在过去的十年中,铜绿假单胞菌噬菌体已被广泛研究为替代抗菌剂。该研究的目的是确定将亚抑制浓度的庆大霉素,头孢曲松,环丙沙星或多粘菌素b与属于Podoviridae和Siphoviridae家族的铜绿假单胞菌特异性噬菌体相结合的抗菌效果。时间杀伤曲线方法表明,噬菌体和亚抑菌浓度的头孢曲松的组合通常会降低细菌的生长,并且在孵育300分钟后证明了Siphoviridae噬菌体 σ-1的协同作用。头孢曲松应用后检测到的形态变化,导致细胞伸长及其特定的作用方式,似乎是必要的,但不是噬菌体-抗生素协同作用的充分理由。该现象为未来发展由铜绿假单胞菌引起的潜在致命感染的治疗策略提供了机会。

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