In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.

译文

在这项研究中,研究了铜绿假单胞菌独特的narK1K2GHJI呼吸操纵子的氧和硝酸盐对转录的调节以及随后的蛋白表达。在PLAC的控制下,铜绿假单胞菌能够转录nar,随后在有氧条件下无硝酸盐的情况下表达甲基紫精连接的硝酸还原酶活性。通过LacI阻遏物对PLAC的调节使我们能够评估生理全细胞硝酸盐还原过程中氧气的转录和翻译后调节。

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