Rats have an attenuated febrile response to endogenous pyrogen near the term of pregnancy. Given the fundamental role of E-series prostaglandins (PGEs) in mediating the febrile response to blood-borne endogenous pyrogen, the present experiments were carried out to determine whether PGEs increase in the area surrounding the organum vasculosum laminae terminalis (peri-OVLT) of near-term pregnant (P) rats as in nonpregnant (NP) rats after intravenous (iv) administration of recombinant rat interleukin-1beta (rrIL-1beta). Core temperature was measured by telemetry and peri-OVLT interstitial fluid was sampled in 12 NP and 12 P chronically instrumented, Sprague-Dawley rats by microdialysis for determination of total PGEs by radioimmunoassay. Basal core temperatures were higher in NP compared with P rats (NP 37.9 degrees C +/- 0.5, P 36.9 degrees C +/- 0.4; P < 0.05), but basal peri-OVLT PGEs were similar in both groups (NP 260 +/- 153 pg/ml, P 278 +/- 177 pg/ml; P =not significant). Intravenous administration of rrIL-1beta to NP rats produced a significant increase in core temperature with a latency, magnitude, and duration of 10 min, 0.87 degrees C, and at least 170 min, respectively; peri-OVLT PGEs were increased significantly by 30 min and averaged 270% above basal levels throughout the experiment. In P rats, however, neither core temperature nor peri-OVLT PGEs increased significantly after iv administration of rrIL-1beta. Intravenous administration of vehicle did not significantly alter core temperature or peri-OVLT PGEs in either group of rats. Thus peri-OVLT PGEs do not increase in P rats as they do in NP rats after iv administration of rrIL-1beta. The mechanism of this interesting component of the maternal adaptation to pregnancy, which likely plays a major role in mediating the attenuated febrile response to endogenous pyrogen near the term of pregnancy, warrants further investigation.

译文

大鼠在怀孕期间对内源性热原的发热反应减弱。鉴于E系列前列腺素 (PGEs) 在介导对血源性内源性热原的发热反应中的基本作用,进行了本实验,以确定在静脉内 (iv) 施用重组大鼠后,近期怀孕 (P) 大鼠的器官脉管终层 (peri-OVLT) 周围区域的PGEs是否增加 (rrIL-1beta)。大鼠interleukin-1beta)。通过遥测法测量核心温度,并通过微透析在12 NP和12 P长期仪器的Sprague-Dawley大鼠中采样周围OVLT间质液,以通过放射免疫测定法测定总PGEs。与P大鼠相比,NP的基础核心温度更高 (NP 37.9 ℃/- 0.5,P 36.9 ℃/- 0.4; P <0.05),但两组的基础peri-OVLT PGEs相似 (NP 260/- 153 pg/ml,P 278 +/- 177 pg/ml; P = 不显着)。向NP大鼠静脉内施用rrIL-1beta会产生核心温度的显着增加,潜伏期,幅度和持续时间分别为10分钟,0.87摄氏度和至少170分钟; peri-OVLT PGEs显着增加30分钟,并且在整个实验中平均270% 高于基础水平。然而,在P大鼠中,静脉注射rrIL-1beta后,核心温度和周围OVLT PGEs均未显着增加。静脉内施用媒介物不会显着改变两组大鼠的核心温度或周围OVLT PGEs。因此,在iv给药rrIL-1beta后,P大鼠中的peri-OVLT PGEs不会像在NP大鼠中那样增加。孕产妇适应妊娠的这一有趣成分的机制可能在介导妊娠期间对内源性热原的减弱的发热反应中起主要作用,值得进一步研究。

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