This paper describes the distribution of NAD+-dependent 15-hydroxy prostaglandin dehydrogenase (delta13-reductase) in the mammalian brain and eye tissues. In addition, an NADH-dependent 15-ketoprostaglandin delta13-reductase (15-PGDH) activity was determined in the brain and eye tissue of some species. The rabbit brain and eye tissues were obtained after arterial perfusion with PBS buffer while the monkey, bovine and porcine tissues were obtained without any treatment. [3H]-PGF2alpha or [3H]-15-keto-17-phenyl-18,19,20-trinor-PGF2alpha1-1-isopropy l ester was incubated with different eye tissue preparations under various conditions. No 15-PGDH activity was observed in the monkey brain while 14% of exogenous PGF2alpha was metabolized to its 15-keto-13,14-dihydro-metabolite by the porcine brain. The 15-PGDH activity in the monkey eye tissue was undetectable. Both brain and different eye tissues hydrolysed 15-keto-17-phenyl-18,19,20-trinor-PGF2alpha-1-isopropyl ester to its free acid and the free acid was further metabolized to 15-keto-13,14-dihydro-17-phenyl-18,19,20-trinor-PGF2alpha by the reduction of 13,14-double bond indicating the presence of a delta13-reductase in these tissues. These data suggest that besides the presence of esterases the mammalian brain and eye tissues possess variable amounts of delta13-reductase in spite of their low 15-PGDH activity which may be of importance in physiology and drug metabolism of these tissues.

译文

本文描述了NAD依赖性15-羟基前列腺素脱氢酶 (delta13-reductase) 在哺乳动物脑和眼组织中的分布。此外,在某些物种的大脑和眼睛组织中确定了NADH依赖性15-酮前列腺素delta13-reductase (15-PGDH) 活性。用PBS缓冲液进行动脉灌注后获得兔脑和眼组织,而无需任何处理即可获得猴,牛和猪组织。[3H]-PGF2alpha或 [3H]-15-酮-17-苯基-18,19,20-trinor-pgf2alpha1-1-异丙基l酯与不同的眼组织制剂在各种条件下孵育。在猴脑中没有观察到15-pgdh活性,而14% 的外源pgf2α 被猪脑代谢为其15-keto-13,14-二氢代谢物。无法检测到猴眼组织中的15-pgdh活性。大脑和不同的眼睛组织都将15-keto-17-phenyl-18,19,20-trinor-pgf2α-1-异丙酯水解为其游离酸,并且游离酸进一步代谢为15-keto-13,14-二氢-17-苯基-18,19,20-trinor-PGF2alpha通过减少13,14-双键表明在这些组织中存在delta13-reductase。这些数据表明,除了酯酶的存在外,哺乳动物的大脑和眼睛组织具有可变量的delta13-reductase,尽管它们的15-pgdh活性较低,这可能在这些组织的生理和药物代谢中很重要。

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