Lignin based materials and chemicals with outstanding sustainability have drawn increasingly attentions. However, the dark color of lignin limits the utilization in color-depended fields. In this work, the factors that influence the color of lignin were investigated and mechanisms were illustrated by GPC, NBO, 2D HSQC, XPS, SEM, and visible light spectrum. It is found that the condensed structures were mainly separated at higher pH due to its high molecular weight and low solubility. The condensation contributes to the conjugation and unsaturation, which resulted in the dark-color of the lignin precipitated at high pH value. The oxidation is not crucial for the color darkening of lignin in drying, it is the micro aggregation that dominantly determined the color degree. The concentration of chromophore was decreased owing to the decrease of bulk density (caused by the alleviation of aggregation), which endowed lignin with the bright seeing macroscopically. Notably, the selection of light-colored lignin needs to be individually considered regarding different use, since the dominating factors that influence the color at solid or solution are totally different. In summary, this work offers guidance for acquiring light-colored lignin and helps people select the light-colored lignin properly regarding utilizations.