Exosomes are key mediators of intercellular communication and play a role in the pathogenesis and progression of cancer. Exosomes in circulating body fluids serve as molecular markers for cancer diagnosis. This study aimed to investigate the role of exosomal microRNA (miR)-1910-3p in breast cancer and determine its clinical diagnostic value. MiR-1910-3p promoted proliferation and migration of breast cancer cells in vitro and in vivo. In vitro, exosomes enriched in miR-1910-3p transferred miR-1910-3p to mammary epithelial cells and breast cancer cells, promoting proliferation and migration, inhibiting apoptosis, and inducing autophagy. In vivo, exosomes enriched in miR-1910-3p promoted the proliferation and migration of breast cancer cells. MiR-1910-3p downregulated myotubularin-related protein 3, activated the NF-κB and wnt/β-catenin signaling pathway, and promoted breast cancer progression. Serum miR-1910-3p in exosomes was an effective diagnostic marker that improved the sensitivity of breast cancer diagnosis when used in combination with the traditional tumor marker CA153. In conclusion, breast cancer cell-derived exosomes promoted the growth, metastasis, and autophagy of breast cancer cells by transferring miR-1910-3p. MiR-1910-3p in serum exosomes may serve as a novel molecular marker for breast cancer diagnosis.

译文

外泌体是细胞间通讯的关键介质,在癌症的发病机理和进展中起作用。循环体液中的外泌体可作为癌症诊断的分子标记。本研究旨在探讨外体microRNA (miR)-1910-3p在乳腺癌中的作用,并确定其临床诊断价值。MiR-1910-3p在体外和体内促进了乳腺癌细胞的增殖和迁移。在体外,富含miR-1910-3p的外泌体miR-1910-3p转移至乳腺上皮细胞和乳腺癌细胞,促进增殖和迁移,抑制细胞凋亡,诱导自噬。在体内,富含miR-1910-3p的外泌体促进了乳腺癌细胞的增殖和迁移。MiR-1910-3p下调肌管蛋白相关蛋白3,激活NF-κ b和wnt/β-catenin信号通路,促进乳腺癌进展。外泌体中的血清miR-1910-3p是与传统肿瘤标志物ca153联合使用时提高乳腺癌诊断敏感性的有效诊断标志物。总之,乳腺癌细胞来源的外泌体通过转移miR-1910-3p促进乳腺癌细胞的生长,转移和自噬。血清外泌体中的MiR-1910-3p可作为乳腺癌诊断的新型分子标志物。

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