The thymic microenvironment plays a key role in the intrathymic T-cell differentiation. It is composed of a tridimensional network of epithelial cells whose physiology is controlled by extrinsic circuits such as neuroendocrine axes. Herein we show that the expression of extracellular matrix ligands and receptor by cultured thymic epithelial cells is upregulated by prolactin (PRL) and growth hormone (GH), the latter apparently occurring via insulin-like growth factor I (IGF-I). Thymocyte release from the lymphoepithelial complexes, thymic nurse cells, as well as the reconstitution of these complexes are enhanced by PRL, GH or IGF-I. Treatment of a mouse thymic epithelial cell line with these hormones induced an increase in thymocyte adhesion, an effect significantly prevented in the presence of antibodies to fibronectin, laminin or respective receptors VLA-5 and VLA-6. Our data suggest that the in vitro changes in thymocyte/thymic epithelial cell interactions induced by pituitary hormones are partially mediated by the enhancement of extracellular matrix ligands and receptors.

译文

胸腺微环境在胸腺内T细胞分化中起关键作用。它由上皮细胞的三维网络组成,其生理受外部回路 (例如神经内分泌轴) 控制。在本文中,我们显示培养的胸腺上皮细胞的细胞外基质配体和受体的表达被催乳素 (PRL) 和生长激素 (GH) 上调,后者显然通过胰岛素样生长因子I (igf-i) 发生。PRL,GH或igf-i增强了从淋巴上皮复合物,胸腺护士细胞的胸腺细胞释放以及这些复合物的重建。用这些激素处理小鼠胸腺上皮细胞系诱导胸腺细胞粘附的增加,在存在针对纤连蛋白,层粘连蛋白或相应受体VLA-5和VLA-6的抗体的情况下,这种作用被显着阻止。我们的数据表明,垂体激素诱导的胸腺细胞/胸腺上皮细胞相互作用的体外变化部分是由细胞外基质配体和受体的增强介导的。

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