Given the heterogeneous nature of multiple sclerosis (MS), we applied DNA microarray technology to determine whether variability is reflected in peripheral blood (PB) cells. In this study, we studied whole-blood gene expression profiles of 29 patients with relapsing-remitting MS (RRMS) and 25 age- and sex-matched healthy controls. We used microarrays with a complexity of 43K cDNAs. The data were analyzed using sophisticated pathway-level analysis in order to provide insight into the deregulated peripheral immune response programs in MS. We found a remarkable elevated expression of a spectrum of genes known to be involved in immune defense in the PB of MS patients compared to healthy individuals. Cluster analysis revealed that the increased expression of these genes was characteristic for approximately half of the patients. In addition, the gene signature in this group of patients was comparable with a virus response program. We conclude that the transcriptional signature of the PB cells reflects the heterogeneity of MS and defines a sub-population of RRMS patients, who exhibit an activated immune defense program that resembles a virus response program, which is supportive for a link between viruses and MS.

译文

鉴于多发性硬化症 (MS) 的异质性,我们应用DNA微阵列技术来确定变异性是否反映在外周血 (PB) 细胞中。在这项研究中,我们研究了29例复发缓解型MS (RRMS) 患者和25例年龄和性别匹配的健康对照者的全血基因表达谱。我们使用了复杂的43K cdna的微阵列。使用复杂的途径水平分析对数据进行分析,以便深入了解MS中放松调节的外周免疫反应程序。与健康个体相比,我们发现MS患者PB中已知参与免疫防御的一系列基因的表达显着升高。聚类分析显示,这些基因的表达增加是大约一半患者的特征。此外,这组患者的基因签名与病毒应答程序相当。我们得出的结论是,PB细胞的转录特征反映了MS的异质性,并定义了RRMS患者的亚群,他们表现出类似于病毒应答程序的激活免疫防御程序,这支持了病毒es和MS之间的联系。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录