The nature of the primary genetic defects in ob/ob and db/db mice are unknown. Both the obese (ob) and diabetes (db) mutations produce similar, multicomponent obese-hyperinsulinemic syndromes when maintained in the same strain of mouse. In an attempt to find differences between these mutations in neuroendocrine function affecting the islets of Langerhans or the pituitary, tissue content of four neuropeptides that are known to be capable of influencing the rate of insulin secretion was examined in obese (ob/ob) and diabetes (db/db) mice. In the first study, C57BL/6Job/ob and control males were studied at 3, 4, and 11 weeks of age. In the second study, db/db mice of both sexes and two inbred strains (C57BL/6J and C57BL/KsJ), which differ markedly in the severity of expression of the diabetes phenotype, were studied at 3 weeks of age, before the development of hyperglycemia and secondary consequences thereof. Immunoreactive peptides were measured in acetic acid extracts of pancreas and pituitary. No differences between male ob/ob and db/db mice of the C57BL/6J strain were found. Marked sex differences in lean control mice were found at 3 weeks of age in pancreatic Met-enkephalin-LI and galanin-LI (with two- to threefold higher content in males). Low pancreatic content (50% to 70% lower than in control mice) of galanin-LI, Met-enkephalin-LI and Leu-enkephalin-LI was associated with hyperinsulinemia in male B6 ob/ob and db/db mice at 3 weeks of age, though not in B6 db/db females and not in BKs db/db mice of either sex.(ABSTRACT TRUNCATED AT 250 WORDS)

译文

ob/ob和db/db小鼠的主要遗传缺陷的性质未知。当维持在相同的小鼠品系中时,肥胖 (ob) 和糖尿病 (db) 突变都会产生相似的多成分肥胖-高胰岛素血症综合征。为了寻找影响朗格汉斯或垂体胰岛的神经内分泌功能的这些突变之间的差异,在肥胖 (ob/ob) 和糖尿病 (db/db) 小鼠中检查了四种已知能够影响胰岛素分泌速率的神经肽的组织含量。在第一项研究中,在3、4和11周龄对C57BL/6Job/ob和对照男性进行了研究。在第二项研究中,研究了两种性别和两种近交系 (C57BL/6J和C57BL/KsJ) 的db/db小鼠,它们在糖尿病表型表达的严重程度上明显不同,在3周龄之前发生高血糖及其继发后果。在胰腺和垂体的乙酸提取物中测量了免疫反应性肽。在C57BL/6J品系的雄性ob/ob和db/db小鼠之间没有发现差异。在3周龄时,在胰腺Met-脑啡肽-LI和galanin-LI中发现瘦对照小鼠的明显性别差异 (男性含量高2至3倍)。galanin-LI,Met-脑啡肽-LI和Leu-脑啡肽-LI的低胰腺含量 (50% 70% 低于对照小鼠) 与3周龄雄性B6 ob/ob和db/db小鼠的高胰岛素血症有关,虽然不是在B6 db/db雌性中,也不是在BKs db/db小鼠中。(摘要截断在250个单词)

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