The ovarian steroid hormone estrogen (E2) elicits a multiplicity of both systemic and uterotropic responses in vivo. For example, the administration of E2 to ovariectomized (Ovx) and sexually immature rodents leads to uterine-specific inflammatory infiltrates. In this study, we quantitated the number of eosinophils and BM8+, Ia+, and CD4+ cells in uteri obtained from adult Ovx control and E2-treated C57BL/6J, C3H/HeJ, and (C57BL/6J x C3H/HeJ) (B6C3) F1 hybrid mice. All three strains exhibited a significant increase in the number of uterine eosinophils and BM8+ macrophages after E2 treatment. However, C57BL/6J and B6C3 F1 hybrid mice responded with a greater number of infiltrating eosinophils and macrophages as compared with C3H/HeJ. A similar analysis of Ia+ and CD4+ cells showed that E2 treatment either down-regulates or does not affect the number of such cells in all three strains. Genome exclusion mapping using a (C57BL/6J x C3H/HeJ) x C3H/HeJ backcross population localized Est1, the major locus controlling the number of eosinophils infiltrating the uterus after E2 treatment, to chromosome 4. In addition, suggestive linkage to marker loci on chromosomes 10 and 16 was detected and evidence for locus interaction is presented. Our results conclusively demonstrate that E2-regulated/ dependent responses can be genetically controlled, indicating that the phenotypic variation observed in both the normal and pathological effects of E2 may, in part, be due to a genetic component.

译文

卵巢类固醇激素雌激素 (E2) 在体内引起多种全身和子宫性反应。例如,向卵巢切除 (Ovx) 和性不成熟的啮齿动物施用E2会导致子宫特异性炎症浸润。在这项研究中,我们定量了从成年Ovx对照和E2-treated C57BL/6J,C3H/HeJ和 (C57BL/6J x C3H/HeJ) (B6C3) F1杂交小鼠获得的子宫中嗜酸性粒细胞和BM8,Ia和CD4细胞的数量。E2处理后,所有这三种菌株的子宫嗜酸性粒细胞和BM8巨噬细胞数量均显着增加。然而,与C3H/HeJ相比,C57BL/6J和B6C3 F1杂交小鼠的浸润嗜酸性粒细胞和巨噬细胞数量更多。对Ia和CD4细胞的类似分析表明,E2处理下调或不影响所有三种菌株中此类细胞的数量。使用 (C57BL/6J x C3H/HeJ) x C3H/HeJ回交群体的基因组排斥图谱将Est1定位为4号染色体,Est1是控制E2治疗后子宫浸润的嗜酸性粒细胞数量的主要基因座。此外,还检测到与10号和16号染色体上的标记基因座的暗示连锁,并提供了基因座相互作用的证据。我们的结果最终证明了E2-regulated/依赖性反应可以在遗传上控制,这表明在E2的正常和病理作用中观察到的表型变异可能部分归因于遗传成分。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录