Deinococcus radiodurans is inherently resistant to both ionizing radiation and desiccation. Fifteen months of desiccation was found to be the LD50 dose for D. radiodurans. Desiccated cells of D. radiodurans entered 6h of growth arrest during post-desiccation recovery (PDR). Proteome dynamics during PDR were mapped by resolving cellular proteins by 2-dimensional gel electrophoresis coupled with mass spectrometry. At least 41 proteins, represented by 51 spots on proteome profiles, were differentially expressed throughout PDR. High upregulation in expression was observed for DNA repair proteins involved in single strand annealing (DdrA and DdrB), nucleotide excision repair (UvrA and UvrB), homologous recombination (RecA) and other vital proteins that contribute to DNA replication, recombination and repair (Ssb, GyrA and GyrB). Expression of CRP/FNR family transcriptional regulator (Crp) remained high throughout PDR. Other pathways such as cellular detoxification, protein homeostasis and metabolism displayed both, moderately induced and repressed proteins. Functional relevance of proteomic modulations to surviving desiccation stress is discussed in detail. Comparison of our data with the published literature revealed convergence of radiation and desiccation stress responses of D. radiodurans. This is the first report that substantiates the hypothesis that the radiation stress resistance of D. radiodurans is incidental to its desiccation stress resistance.

译文

放射性球菌对电离辐射和干燥具有固有的抵抗力。发现15个月的干燥是D. radiodurans的LD50剂量。在干燥后恢复 (PDR) 期间,放射性D. radiodurans的干燥细胞进入6小时的生长停滞。PDR期间的蛋白质组动力学通过二维凝胶电泳结合质谱解析细胞蛋白来定位。在整个PDR中,至少有41种蛋白质 (由蛋白质组谱上的51个斑点表示) 差异表达。观察到参与单链退火 (DdrA和DdrB),核苷酸切除修复 (UvrA和UvrB),同源重组 (reea) 和其他有助于DNA复制,重组和修复的重要蛋白的DNA修复蛋白的表达高度上调 (Ssb,GyrA和GyrB)。在整个PDR中,CRP/FNR家族转录调节因子 (Crp) 的表达仍然很高。其他途径,例如细胞解毒,蛋白质稳态和代谢,同时显示出中度诱导和抑制的蛋白质。详细讨论了蛋白质组学调节与存活的干燥胁迫的功能相关性。将我们的数据与已发表的文献进行比较,发现D. radiodurans的辐射和干燥应力响应会收敛。这是第一份证实以下假设的报告: D. radiodurans的辐射应力性与其干燥应力性有关。

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