We studied the roles of Notch, Delta, and Serrate in vertebrate epithelial appendage morphogenesis using feather as a model and found the following. (1) C-Notch-1, C-Delta-1, and C-Serrate-1 are not expressed at the early placode stage and are therefore not involved in the determination of bud versus interbud compartments. (2) From symmetric short buds to asymmetric long buds, C-Delta-1 and C-Serrate-1 are expressed in the posterior bud mesenchyme in a nested fashion, while C-Notch-1 is expressed as a stripe perpendicular to the anterior-posterior (A-P) axis and positioned posterior to the midpoint. (3) Epithelial-mesenchymal recombination with rotation led to the disappearance of these genes followed by their reappearance with new positions appearing to predict their new morphological orientation. (4) Conditions leading to branched buds (e.g., recombination of later buds) show polarized staining patterns before branching occurs. (5) Conditions leading to symmetrical round buds (e.g., treated with the protein kinase A agonist forskolin) suppress expression of all three genes. These results lead us to hypothesize that Notch, Delta, and Serrate are involved in establishing the A-P asymmetry of feather buds.

译文

我们以羽毛为模型研究了Notch,Delta和Serrate在脊椎动物上皮附件形态发生中的作用,发现以下内容。(1) C-Notch-1,C-Delta-1和C-Serrate-1在早期阶段不表达,因此不参与芽间隔室的确定。(2) 从对称的短芽到不对称的长芽,C-Delta-1和C-Serrate-1以嵌套方式在后芽间充质中表达,而C-Notch-1表示为垂直于前后 (a-P) 轴并位于后的条纹中点。(3) 旋转使上皮-间充质重组导致这些基因消失,然后以新的位置重新出现,以预测其新的形态方向。(4) 导致分支芽的条件 (例如,后期芽的重组) 在分支发生之前显示出极化染色模式。(5) 导致对称圆芽的条件 (例如,用蛋白激酶A激动剂forskolin处理) 抑制所有三个基因的表达。这些结果使我们假设缺口,三角洲和锯齿参与建立羽毛芽的A-P不对称性。

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