A small cryptic Lactobacillus helveticus plasmid, pLBL4, was able to reveal restriction fragment length polymorphism in different bacterial species including Lactobacillus species, Bacillus species, and Escherichia coli when used as a DNA probe. The observed polymorphism was a result of the combined hybridization of several microsatellite sequences. The 6-bp sequence (TTGTTT) was repeated 12 times, seven of which were concentrated within the region between 1791 and 1997 bp of the plasmid sequence. The polymorphic patterns generated with pLBL4 differed from those obtained with M13 DNA in the larger number of bands observed. The results presented here open the possibility of using pLBL4 as a new broad-spectrum polymorphic DNA probe for fingerprint analysis.

译文

当用作DNA探针时,一个小的隐秘螺旋乳杆菌质粒pLBL4能够揭示不同细菌物种 (包括乳杆菌物种,芽孢杆菌物种和大肠杆菌) 中的限制性片段长度多态性。观察到的多态性是几个微卫星序列联合杂交的结果。将6-bp序列 (TTGTTT) 重复12次,其中7次集中在质粒序列的1791至1997 bp之间的区域内。在观察到的大量条带中,pLBL4生成的多态性模式与M13 DNA获得的多态性模式不同。此处介绍的结果为使用pLBL4作为新的广谱多态性DNA探针进行指纹分析提供了可能性。

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