Fractionation of rat L6 myoblast histone H4 mRNA into its three component subspecies revealed that one of the major subspecies (H4-1) contained poly(A). The unique poly(A)+ H4 mRNA makes up about 8% of the total polysomal H4 mRNA population detected. Unlike the poly(A)- histone mRNAs, whose levels are reduced by greater than 95% when myoblasts differentiate into myotubes, the poly(A)+ subspecies is reduced by only 70%. The poly(A)+ H4 mRNA from myotubes incubated with actinomycin D decays with a half-life of 37-42 min, which is similar to that obtained for the poly(A)- H4 mRNAs in myoblasts. Both the poly(A)+ and poly(A)- subspecies decay at an increased rate after inhibition of DNA synthesis. In myoblasts the poly(A)+ H4 mRNA exists almost exclusively in the polysomal compartment (greater than 95%) with little (less than 5%) in the free ribonucleoprotein (mRNA-protein or mRNP) complex compartment of the cell. Poly(A)- histone H4 mRNA subspecies, on the other hand, are distributed with approximately 80% in the polysomal compartment and 20% in the free mRNP complex compartment. The unique poly(A)+ H4 mRNA is unusual, not only in that it contains poly(A) but also in its behavior compared to poly(A)- H4 mRNAs during terminal differentiation.

译文

将大鼠L6成肌细胞组蛋白H4 mRNA分离成其三个组成亚种,表明主要亚种 (H4-1) 之一含有poly(A)。独特的poly(A)+ h4mrna构成了检测到的总多体h4mrna群体的约8%。与当成肌细胞分化为肌管时其水平降低大于95% 的聚 (A)-组蛋白mrna不同,聚 (A) 亚种仅降低70%。与放线菌素d孵育的肌管中的poly(A) H4 mRNA衰变,半衰期为37-42分钟,这与在成肌细胞中获得的poly (a)- H4 mRNA相似。在抑制DNA合成后,poly(A) 和聚 (A) 亚种均以增加的速率衰减。在成肌细胞中,poly(A) h4mrna几乎仅存在于多体区室 (大于95%) 中,很少 (小于5%) 存在于细胞的游离核糖核蛋白 (mRNA-蛋白或mRNP) 复合区室中。另一方面,聚 (A)-组蛋白h4mrna亚种在多体区室中分布有大约80%,而在游离的mRNP复合物区室中20%。独特的poly(A) H4 mRNA是不寻常的,不仅因为它包含poly(A),而且在末端分化过程中与poly(A)- H4 mRNA相比,其行为也是如此。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录