The time course of ethylene biosynthesis and perception was investigated in ripening peach fruit (Prunus persica) following treatments with the polyamines putrescine (Pu) and spermidine (Sd), and with aminoethoxyvinylglycine (AVG). Fruit treatments were performed in planta. Ethylene production was measured by gas chromatography, and polyamine content by high-performance liquid chromatography; expression analyses were performed by Northern blot or real-time polymerase chain reaction. Differential increases in the endogenous polyamine pool in the epicarp and mesocarp were induced by treatments; in both cases, ethylene production, fruit softening and abscission were greatly inhibited. The rise in 1-aminocyclopropane-1-carboxylate oxidase (PpACO1) mRNA was counteracted and delayed in polyamine-treated fruit, whereas transcript abundance of ethylene receptors PpETR1 (ethylene receptor 1) and PpERS1 (ethylene sensor 1) was enhanced at harvest. Transcript abundance of arginine decarboxylase (ADC) and S-adenosylmethionine decarboxylase (SAMDC) was transiently reduced in both the epicarp and mesocarp. AVG, here taken as a positive control, exerted highly comparable effects to those of Pu and Sd. Thus, in peach fruit, increasing the endogenous polyamine pool in the epicarp or in the mesocarp strongly interfered, both at a biochemical and at a biomolecular level, with the temporal evolution of the ripening syndrome.

译文

在用多胺腐胺 (Pu) 和亚精胺 (Sd) 以及氨基乙氧基乙烯基甘氨酸 (AVG) 处理后,研究了成熟桃果实 (Prunus persica) 中乙烯生物合成和感知的时间过程。在植物中进行水果处理。通过气相色谱法测量乙烯产量,通过高效液相色谱法测量多胺含量; 通过Northern印迹或实时聚合酶链反应进行表达分析。处理可诱导外皮和中果皮中内源多胺库的差异增加; 在这两种情况下,乙烯的产生,果实的软化和脱落均受到极大抑制。在多胺处理的水果中,1-氨基环丙烷-1-羧酸氧化酶 (PpACO1) mRNA的上升被抵消并延迟,而乙烯受体PpETR1 (乙烯受体1) 和pper1 (乙烯传感器1) 的转录物丰度在收获时增强。精氨酸脱羧酶 (ADC) 和S-腺苷甲硫氨酸脱羧酶 (SAMDC) 的转录物丰度在表皮和中果皮中均瞬时降低。AVG作为阳性对照,与Pu和Sd具有高度可比的作用。因此,在桃子果实中,在生化和生物分子水平上,内源性多胺库的增加会强烈干扰成熟综合征的时间演变。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录