The membranotropic properties of block co-polymers and their protein conjugates were studied by their effect on the rate of oxygen consumption by isolated liver mitochondria and on thymus-derived lymphocytes. The block co-polymers consisted of poly(ethylene oxide) (PoE) [poly(ethylene glycol)] and poly(propylene oxide) (PoP) to give either PoE-PoP or PoE-PoP-PoE. Both types inhibited uncoupled respiration of liver mitochondria in a medium containing glutamate and malate and also of lymphocytes. They also uncoupled respiration in the presence of succinate in K(+)-containing medium and of lymphocytes. A method is described for linking protein to the block polymers to form conjugates. Such conjugates were formed from alpha-chymotrypsin, BSA and cytochrome c, all of which produced similar effects on the respiration of the isolated mitochondria and lymphocytes. The data suggest that both the block co-polymers and their protein conjugates inhibit the NADH dehydrogenase complex and induce a K(+)-conductivity of the mitochondrial inner membrane; the surface activity of the conjugates allows them to pass through the plasma membrane and interact with the mitochondrial inner membrane.

译文

通过对分离的肝线粒体和胸腺来源的淋巴细胞耗氧率的影响,研究了嵌段共聚物及其蛋白质缀合物的膜致特性。嵌段共聚物由聚 (环氧乙烷) (PoE) [聚 (乙二醇)] 和聚 (环氧丙烷) (PoP) 组成,从而得到PoE-PoP或PoE-PoP-PoE。两种类型均抑制含有谷氨酸和苹果酸以及淋巴细胞的培养基中肝线粒体的未偶联呼吸。在含K () 的培养基和淋巴细胞中存在琥珀酸盐的情况下,它们还会解耦呼吸。描述了一种用于将蛋白质连接到嵌段聚合物以形成缀合物的方法。这种结合物是由 α-胰凝乳蛋白酶,BSA和细胞色素c形成的,所有这些结合物对分离的线粒体和淋巴细胞的呼吸产生相似的作用。数据表明,嵌段共聚物及其蛋白质缀合物均抑制NADH脱氢酶复合物并诱导线粒体内膜的K () 电导率; 缀合物的表面活性使它们能够穿过质膜并与线粒体内膜相互作用。

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