A novel mycovirus was isolated from a cultivated edible mushroom, Pleurotus eryngii, with severe epidemic symptoms. Purification of the virus was carried out by a sequential procedure of polyethylene glycol precipitation, differential centrifugation, and equilibrium centrifugation in a CsCl gradient. Nuclease digestion assay and protein analysis revealed that the virus consisted of a single-stranded RNA (ssRNA) genome of 7.8 kbp which was encapsulated by a coat protein of 22 kDa. Transmission electron microscope showed that it was spherical with a diameter of 31 nm. Since there was neither a previous report on discovery of a virus in P. eryngii, nor known mushroom viruses with similar characteristics, we concluded that this is a novel virus and thus have named it as P. e ryngii Spherical Virus (PeSV). Because of a diagnostic test would be helpful in preventing the PeSV-related disease outbreaks, we developed a triple antibody sandwich-ELISA (TAS-ELISA) system using anti-PeSV mouse monoclonal and anti-PeSV rabbit polyclonal antibodies. The TAS-ELISA system successfully detected less than 0.5 microg of the virus particles in 1 g diseased mushroom tissue collected from various commercial farms.

译文

从栽培的食用蘑菇杏鲍菇中分离出一种新型分枝杆菌,具有严重的流行症状。病毒的纯化是通过在CsCl梯度中进行的聚乙二醇沉淀,差速离心和平衡离心的顺序程序进行的。核酸酶消化试验和蛋白质分析表明,该病毒由7.8 kbp的单链RNA (ssRNA) 基因组组成,该基因组被22 kDa的外壳蛋白包裹。透射电子显微镜显示它是球形的,直径为31 nm。由于以前没有关于在杏鲍菇中发现病毒的报道,也没有已知具有类似特征的蘑菇病毒,因此我们得出结论,这是一种新型病毒,因此将其命名为杏鲍菇球形病毒 (PeSV)。由于诊断测试有助于预防PeSV相关疾病的爆发,因此我们开发了使用抗PeSV小鼠单克隆和抗PeSV兔多克隆抗体的三重抗体夹心ELISA (TAS-ELISA) 系统。TAS-ELISA系统成功地检测到从各种商业农场收集的1 g患病蘑菇组织中少于0.5微克的病毒颗粒。

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