The novelty of the present work looks in the synthesis of aqueous dispersed selenium nanoparticles (Se NPs) using gamma rays with the aid of various natural macromolecules such as citrus pectin (CP), sodium alginate (Alg), chitosan (CS) and aqueous extract of fermented fenugreek powder (AEFFP) using Pleurotus ostreatus for investigating their impact in vitro toward carcinoma cell. The synthesized Se NPs were characterized by XRD, UV-Vis., DLS, HRTEM, SEM, EDX and FTIR. Nucleation and growth mechanisms were also discussed. The factorial design was applied to examine the importance of multiple parameters on Se NPs production with a special focus on temperature and gamma rays influences. FTIR spectrum exhibited the existence of several functional groups in Se NPs-capping macromolecules. Results revealed that Se NPs' size was dramatically-influenced by the type of stabilizer, precursors concentration, pH and the absorbed gamma rays dose. The current research reported the promising antitumor application of Se NPs against Ehrlich Ascites Carcinoma (EAC) and human Colon Adenocarcinoma (CACO) in vitro. The proliferation of EAC was significantly-hindered by Se NPs-CS (38.0 μg/ml) at 60 kGy (IC50 = 23.12%) and Se NPs-AEFFP (19.00 μg/ml) at 15 kGy (IC50 = 7.21%). Also, Se NPs control the generation of CACO cells, IC50 was recorded as 25.32% for Se NPs-CS (38.0 μg/ml) and 8.57% for Se NPs-AEFFP (19.00 μg/ml).

译文

本工作的新颖之处在于使用 γ射线在各种天然大分子 (例如柑橘果胶 (CP),海藻酸钠 (Alg)) 的帮助下合成水性分散的硒纳米颗粒 (Se NPs),壳聚糖 (CS) 和发酵胡芦巴粉 (AEFFP) 的水提取物使用平菇研究其对癌细胞的体外影响。通过XRD,UV-Vis,DLS,HRTEM,SEM,EDX和FTIR对合成的Se NPs进行了表征。还讨论了成核和生长机理。析因设计用于检查多个参数对Se NPs生产的重要性,特别关注温度和伽马射线的影响。FTIR光谱显示Se NPs封端大分子中存在几个官能团。结果表明,Se np的大小受稳定剂类型,前体浓度,pH和吸收的伽马射线剂量的影响很大。目前的研究报道了Se NPs在体外对艾氏腹水癌 (EAC) 和人结肠腺癌 (CACO) 的抗肿瘤应用前景。60 kGy (IC50 = 23.12%) 的Se NPs-CS (38.0 μ g/ml) 和15 kGy (IC50 = 7.21%) 的Se NPs-AEFFP (19.00 μ g/ml) 显着阻碍了EAC的增殖。此外,Se NPs控制CACO细胞的产生,IC50被记录为Se nps-cs (38.0 μ g/ml) 的25.32% 和Se nps-aeffp (19.00 μ g/ml) 的8.57%。

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