Auto-anti-idiotypic mechanisms can regulate the protective immune response against Schistosoma mansoni. Anti-idiotypic responses were stimulated by immunization of mice either with nonspecifically induced lymphoblasts, produced with Con A, or with Ag-induced lymphoblasts bearing specific idiotypic receptors. The effect of the induced anti-idiotypic response upon clonotypic cellular reactivity was assessed in vitro through the suppression of antigen-mediated blast transformation by cloned T cells and in vivo by suppression of resistance to S. mansoni and delayed-type hypersensitivity responses against specific Ag. Differential regulation of humoral immune responses was studied at the levels of specific epitopic recognition, the expression of specific Id, and the production of anti-idiotypic responses directed against mAb bearing specific Id. Anti-idiotypic sensitization resulted in variable (10 to 90%) suppression of the immune response to discrete antigenic epitopes, the expression of specific idiotypic phenotypes, and anti-idiotypic, antiparatopic responses against T cell clonotypes and antibody idiotypic phenotypes. In vitro admixture and in vivo challenge studies resulted in consonant differential suppression. Thus idiotypic regulation can mold the fine specificities of the protective immune response to S. mansoni at the clonal level and may provide an approach to optimize the expression and assessment of resistance.

译文

自身抗独特型机制可以调节曼氏血吸虫的保护性免疫反应。通过用Con A产生的非特异性诱导的淋巴母细胞或带有特定独特型受体的Ag诱导的淋巴母细胞免疫小鼠来刺激抗独特型反应。诱导的抗独特型反应对克隆型细胞反应性的影响在体外通过克隆的T细胞抑制抗原介导的胚转化进行评估,并在体内通过抑制对曼氏杆菌的抗性和对特异性Ag的延迟型超敏反应进行评估。在特定的表位识别,特定Id的表达以及针对带有特定Id的mAb的抗独特型反应的产生的水平上研究了体液免疫反应的差异调节。抗独特型致敏作用导致对离散抗原表位的免疫应答、特定独特型表型的表达以及针对T细胞克隆型和抗体独特型表型的抗独特型、抗独特型应答的可变 (10至90%) 抑制。体外混合和体内激发研究导致辅音差异抑制。因此,独特型调节可以在克隆水平上塑造对曼氏杆菌的保护性免疫反应的精细特异性,并可能提供一种优化表达和评估抗性的方法。

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