The polymerase chain reaction has been applied to the detection of Escherichia coli DNA in the upper gut contents of Lindow Man, an Iron Age bog body dated to ca 300 BC. With sets of primers from the uidA and lacZ genes, E. coli DNA could be detected reproducibly. Initial attempts at detecting DNA from freshly voided faeces from a healthy volunteer were unsuccessful due to inhibition of the reaction. Development of a method, based on guanidine thiocyanate and silica extraction and purification of the DNA fragments, facilitated the detection of the E. coli DNA in both freshly voided faeces and the upper gut contents of Lindow Man. These findings indicate that it may be possible to study the existence of infectious diseases in ancient civilizations and to learn more about the evolution of microbes.

译文

聚合酶链反应已用于检测Lindow Man的上肠内容物中的大肠杆菌DNA,Lindow Man是铁器时代的沼泽体,可追溯至公元前300年。使用uidA和lacZ基因的引物组,可以重复检测大肠杆菌DNA。由于抑制反应,最初尝试从健康志愿者的新鲜排空粪便中检测DNA的尝试均未成功。基于硫氰酸胍和二氧化硅提取和纯化DNA片段的方法的开发,有助于检测新鲜排空的粪便和Lindow Man的上肠内容物中的大肠杆菌DNA。这些发现表明,可能有可能研究古代文明中传染病的存在,并更多地了解微生物的进化。

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