In several species, placenta has been found to express GH-related proteins. In the ovine placenta, such a protein, ovine chorionic somatommamotropin, has been described, but its involvement in the fetal/placental growth process is not clearly established. The aim of this study was to investigate the occurrence of another GH-related peptide in the ovine placenta. Placental extracts (days 30-140 of pregnancy) showed GH immunoreactivity between days 35-70. SDS-PAGE analysis of these extracts indicated that this immunoreactivity corresponded to 22- and 28-kDa proteins. GH-like immunoreactivity was localized on cotyledonary frozen sections in the syncytium and the trophectoderm. Northern blot analysis of placental RNA showed the expression of GH-hybridizing transcripts migrating to the same position as that of GH pituitary messenger RNA (mRNA). Those transcripts were highly expressed between days 40 and 50. Their sequence analysis showed the existence of three GH mRNA (GHP1, GHP2, and GHP3). GHP1 is identical to pituitary GH mRNA and probably codes for the 22-kDa protein. GHP2 and GHP3 encode the same protein, which differs from GHP1 by four amino acids. This study establishes the expression of GH gene and GH-immunoreactive proteins in the ovine placenta.

译文

在几个物种中,胎盘被发现表达GH相关蛋白。在绵羊胎盘中,已经描述了这种蛋白质,即绵羊绒毛膜生长激素,但尚未明确其参与胎儿/胎盘生长过程。这项研究的目的是调查绵羊胎盘中另一种GH相关肽的发生。胎盘提取物 (妊娠第30-140天) 在第35-70天之间显示出GH免疫反应性。这些提取物的sdds-PAGE分析表明,该免疫反应性对应于22-和28-kDa蛋白。GH样免疫反应性位于合胞体和滋养外胚层的子叶冷冻切片上。胎盘RNA的Northern印迹分析显示,GH杂交转录本的表达迁移到与GH垂体信使RNA (mRNA) 相同的位置。这些成绩单在第40至50天之间高度表达。他们的序列分析显示存在三种GH mRNA (GHP1,GHP2和GHP3)。GHP1与垂体GH mRNA相同,可能编码22-kda蛋白。GHP2和GHP3编码相同的蛋白质,与GHP1的不同之处在于四个氨基酸。本研究建立了GH基因和GH免疫反应蛋白在绵羊胎盘中的表达。

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