Estrogen receptor antagonists are effective in breast cancer treatment. However, the side effects of these treatments have led to a rise in searching for alternative therapies. The present study evaluated the estrogenic, antiestrogenic, and antiproliferative activities of Euphorbiabicolor (Euphorbiaceae), a plant native to south-central USA. Estrogenic and antiestrogenic activities of latex extract and its phytochemicals were evaluated with a steroid-regulated yeast system expressing the human estrogen receptor α and antiproliferative properties were assessed in the ER-positive MCF-7 and T47-D and triple-negative MDA-MB-231 and MDA-MB-469 breast carcinomas. Genistein and coumestrol identified in the latex extract induced higher estrogenic and antiestrogenic activities compared to diterpenes and flavonoids. The latex extract, resiniferatoxin (RTX) and rutin induced antiproliferative activities in all cell lines in a dose-dependent manner, but not in human normal primary dermal fibroblast cultures. A biphasic effect was observed with MDA-MB-468 breast carcinoma in which the latex extract at low concentrations increased and at high concentrations decreased cell proliferation. Treatments with latex extract in combination with RTX or rutin reduced even more the proliferation of MCF-7 breast carcinoma compared to the individual latex, RTX, and rutin treatments. E. bicolor latex phytochemicals could contribute to developing commercial therapeutic agents for breast cancer treatment.

译文

雌激素受体拮抗剂对乳腺癌治疗有效。然而,这些治疗的副作用导致了寻找替代疗法的增加。本研究评估了原产于美国中南部的大戟科 (Euphorbiaceae) 的雌激素,抗雌激素和抗增殖活性。用表达人类雌激素受体 α 的类固醇调节酵母系统评估了乳胶提取物及其植物化学物质的雌激素和抗雌激素活性,并在ER阳性MCF-7,T47-D和三阴性MDA-MB-231和MDA-MB-469乳腺癌中评估了抗增殖特性。与二萜和类黄酮相比,在乳胶提取物中鉴定的金雀异黄素和香豆酚可诱导更高的雌激素和抗雌激素活性。乳胶提取物,树脂曲霉毒素 (RTX) 和芦丁以剂量依赖性方式在所有细胞系中诱导抗增殖活性,但在人正常的原代真皮成纤维细胞培养物中却没有。在MDA-MB-468乳腺癌中观察到双相效应,其中低浓度的乳胶提取物增加,而高浓度的乳胶提取物降低了细胞增殖。与单独的乳胶、RTX和芦丁治疗相比,乳胶提取物与RTX或芦丁联合治疗甚至更多地降低了MCF-7乳腺癌的增殖。E.双色乳胶植物化学物质可能有助于开发用于乳腺癌治疗的商业治疗剂。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录