The level of cyclic AMP in various fractions of rat skeletal tissue was measured after in vitro or in vivo administration of parathyroid hormone and calcitonin. Incubations of bone fractions prepared from young (5 weeks of age thyroparathyroidectomized rats revealed that both parathyroid hormone and calcitonin increased the cyclic AMP level in fractions of epiphysis, metaphysis and marrow cells. Cyclic AMP accumulation in incubated perisoteum and diaphysis were induced solely by parathyroid hormone. In in vivo experiments the cyclic AMP level in the tibia of the thyroparathyroidectomized rat was increased by infusion of either parathyroid hormone or calcitonin, and the simultaneous administration of each maximally effective dose of the two hormones exhibited an additive effect. Within 2 min, parathyroid hormone infusion caused an elevation of cyclic AMP content in periosteum and metaphysis. Rapid increase of cyclic AMP in the metaphysis was also induced by calcitonin, and the effect of the two hormones on cyclic AMP accumulation in this fraction was additive. Small but significant increase of cyclic AMP in the diaphysis was detected at 5 min after the administration of parathyroid hormone. Calcitonin infusion did not show any consistent effects on periosteum and diaphysis.

译文

在体外或体内给予甲状旁腺激素和降钙素后,测量大鼠骨骼组织各部分中的环AMP水平。从年轻的 (5周龄甲状腺旁腺切除的大鼠) 制备的骨组分的孵育表明,甲状旁腺激素和降钙素均增加了骨骺组分中的环AMP水平,干phy端和骨髓细胞。仅由甲状旁腺激素诱导孵育的perisoteum和daphysis中的循环AMP积累。在体内实验中,甲状旁腺激素或降钙素的输注增加了甲状旁腺切除大鼠胫骨中的循环AMP水平,两种激素的最大有效剂量的同时给药均表现出累加作用。在2分钟内,甲状旁腺激素输注导致骨膜和干骺端循环AMP含量升高。降钙素也诱导了干骺端循环AMP的快速增加,两种激素对该级分中环AMP积累的影响是累加的。甲状旁腺激素给药后5分钟,检测到骨干中环AMP的少量但显着增加。降钙素输注对骨膜和骨干没有显示出任何一致的作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录