Rhodamine 123 (R123) is a permeant, cationic, fluorescent dye that localizes preferentially within mitochondria of living carcinoma cells. MGH-U1 human bladder carcinoma cells incubated in vitro with 10 microM R123 for 30 min and then irradiated at 514.5 nm with an argon ion laser underwent selective, phototoxic injury to mitochondria. Ultrastructurally, treatment with R123 plus irradiation with 10 J/cm2 caused selective, progressive mitochondrial alterations consisting of disruption of cristae, vacuolization, swelling, increasing numbers of ring-shaped and angulated mitochondria at 4 to 8 h after irradiation, and obliteration of many mitochondria at 24 to 48 h. Confocal laser scanning microscopy after treatment with R123 plus irradiation with 10 to 30 J/cm2 demonstrated altered uptake and localization of subsequently administered R123, accompanied by striking mitochondrial fragmentation. Irradiation caused a dose-dependent depletion of extractable R123, due to a photosensitized efflux that began immediately and progressed by 4 h after irradiation with 10 to 30 J/cm2; further uptake after reincubation in the presence of R123 was also quantitatively impaired in cells previously irradiated with 30 J/cm2.

译文

罗丹明123 (R123) 是一种渗透的阳离子荧光染料,优先定位在活癌细胞的线粒体内。MGH-U1人膀胱癌细胞与10 microM R123体外孵育30分钟,然后用氩离子激光在514.5 nm照射,对线粒体进行选择性的光毒性损伤。在超微结构上,用R123加10 J/cm2辐照处理会导致选择性的进行性线粒体改变,包括cr破坏,空泡化,肿胀,照射后4至8小时环形和成角度的线粒体数量增加,以及在24至48小时内许多线粒体消失。用R123加10至30 J/cm2辐照处理后的共聚焦激光扫描显微镜显示,随后施用的R123的摄取和定位发生了改变,并伴有明显的线粒体断裂。辐照导致可提取的R123的剂量依赖性耗竭,这是由于光敏外排立即开始并在用10至30 J/cm2辐照后4小时内进展; 在先前用30 J/cm2辐照的细胞中,在R123存在下重新吸收后的进一步吸收也受到定量损害。

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