Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.

译文

基因编码的光敏剂越来越多地用作控制细胞命运或触发细胞内过程的光遗传学工具。最近开发了一种称为超新星的单体红色荧光蛋白,但是,它在大多数基于光毒性的应用中表现出次优的特性。在这里,我们对该蛋白进行了定向进化,并鉴定了SuperNova2,这是一种具有S10R取代的蛋白,可在细菌和哺乳动物细胞培养物中提高亮度,发色团成熟和光毒性。

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