Chemically and biologically modified nanoparticles are increasingly considered as viable and multifunctional tools to be used in cancer theranostics. Herein, we demonstrate that coordination of alizarin blue black B (ABBB) to the TiO(2) nanoparticle surface enhances the resulting nanoparticles by (1) creating distinct fluorescence emission spectra that differentiate smaller TiO(2) nanoparticles from larger TiO(2) nanoparticle aggregates (both in vitro and intracellular) and (2) enhancing visible light activation of TiO(2) nanoparticles above previously described methods to induce in vitro and intracellular damage to DNA and other targets. ABBB-TiO(2) nanoparticles are characterized through sedimentation, spectral absorbance, and gel electrophoresis. The possible coordination modes of ABBB to the TiO(2) nanoparticle surface are modeled by computational methods. Fluorescence emission spectroscopy studies indicate that ABBB coordination on TiO(2) nanoparticles enables discernment between nanoparticles and nanoparticle aggregates both in vitro and intracellular through fluorescence confocal microscopy. Visible light activated ABBB-TiO(2) nanoparticles are capable of inflicting increased DNA cleavage through localized production of reactive oxygen species as visualized by plasmid DNA damage detected through gel electrophoresis and atomic force microscopy. Finally, visible light excited ABBB-TiO(2) nanoparticles are capable of inflicting damage upon HeLa (cervical cancer) cells by inducing alterations in DNA structure and membrane associated proteins. The multifunctional abilities of these ABBB-TiO(2) nanoparticles to visualize and monitor aggregation in real time, as well as inflict visible light triggered damage upon cancer targets will enhance the use of TiO(2) nanoparticles in cancer theranostics.

译文

化学和生物修饰的纳米颗粒越来越被认为是用于癌症治疗的可行和多功能工具。在此,我们证明了茜素蓝黑B (ABBB) 与TiO(2) 纳米颗粒表面的配位通过 (1) 创建独特的荧光发射光谱,将较小的TiO(2) 纳米颗粒与较大的TiO(2) 纳米颗粒聚集体区分开来 (在体外和细胞内) 和 (2) 增强TiO(2) 纳米颗粒的可见光活化上述方法诱导体外和细胞内损伤DNA和其他靶标。ABBB-TiO(2) 纳米颗粒通过沉降,光谱吸光度和凝胶电泳进行表征。通过计算方法对ABBB与TiO(2) 纳米颗粒表面的可能协调模式进行了建模。荧光发射光谱研究表明,在TiO(2) 纳米颗粒上的ABBB配位能够通过荧光共聚焦显微镜在体外和细胞内识别纳米颗粒和纳米颗粒聚集体。可见光激活的ABBB-TiO(2) 纳米颗粒能够通过通过凝胶电泳和原子力显微镜检测到的质粒DNA损伤来观察,从而通过局部产生活性氧而导致DNA裂解增加。最后,可见光激发的ABBB-TiO(2) 纳米颗粒能够通过诱导DNA结构和膜相关蛋白的改变而对HeLa (宫颈癌) 细胞造成损害。这些ABBB-TiO(2) 纳米颗粒实时可视化和监测聚集以及对癌症靶标造成可见光触发损害的多功能能力将增强TiO(2) 纳米颗粒在癌症治疗中的应用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录