A2E (2-[2,6-dimethyl-8-(2,6,6-trimethyl-1-cyclohexen-1-yl)-1E, 3E,5E,7E-octatetraenyl]-1-(2-hydroxyethyl)-4-[4-methyl-6(2,6,6-trimethyl-1-cyclohexen-1-yl)-1E,3E,5E-hexatrienyl]pyridinium) is a blue-absorbing molecular constituent of human ocular lipofuscin and contributes to the golden-yellow emission of this pigment. Lipofuscin photoproduces toxic reactive oxygen intermediates (ROI), but the specific molecular components responsible for this phototoxicity remain unidentified. In this article the aerobic photoreactivity of A2E is quantified by comparison with its biosynthetic precursor, all-trans-retinal, and with other appropriate standards. Under blue-light exposure the efficacies for formation of cholesterol (Ch) hydroperoxides and the superoxide radical anion (O2*-) were determined using high-pressure liquid chromatography with electrochemical detection and electron spin resonance oximetry and spin trapping, respectively. Photogeneration of singlet oxygen after blue-light excitation of A2E was demonstrated unambiguously by the Ch peroxidation assay. After blue-light irradiation of A2E, O2*- were detected, but the concentration was insufficient to account for the measured production of O2*- by the solvent extract of lipofuscin granules. The collective data support the conclusion that A2E does not produce sufficient concentrations of ROI to be the primary phototoxic constituent of lipofuscin.

译文

A2E (2-[2,6-二甲基-8-(2,6,6-三甲基-1-环己烯-1-基)-1E,3E,5E,7e-八四烯基]-1-(2-羟乙基)-4-[4-甲基-6(2,6,6-三甲基-1-环己烯-1-基)-1E,3E,5e-己三烯基] 吡啶鎓) 是人眼脂褐素的一种吸收蓝色的分子成分,有助于这种色素的金黄色发射。脂褐素光产生有毒的活性氧中间体 (ROI),但是导致这种光毒性的特定分子成分仍然未知。在本文中,A2E的需氧光反应通过与其生物合成前体全反式视网膜进行比较来量化。和其他适当的标准。在蓝光照射下,使用高压液相色谱电化学检测,电子自旋共振血氧饱和度和自旋捕获法测定了胆固醇 (Ch) 氢过氧化物和超氧化物自由基阴离子 (O2 *-) 的形成效率。分别。通过Ch过氧化试验明确证明了A2E在蓝光激发后单线态氧的光生。在A2E的蓝光照射后,检测到O2 *-,但是浓度不足以说明脂褐素颗粒的溶剂提取物测得的O2 *-的产生。集体数据支持以下结论: A2E不能产生足够浓度的ROI作为脂褐素的主要光毒性成分。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录