The growth adaptability to increasing concentration of the biocide 2-phenoxyethanol (PE) was determined in Pseudomonas aeruginosa PAO1 (P.a.) as part of efforts to understand and control the biocide tolerance and its effect on cross-resistance to other biocides and resistance to antibiotics. After repeated subculturing in media containing increasing sub-minimum-inhibitory PE concentration, P.a. exhibited an adaptive resistance indicated by two-fold increase in MIC at the 10th passage. The resistance was stable and remained after passaging the strain in further 7 successive passages in PE-free growth media. The strain showed cross-resistance towards dissimilar biocides and displayed increased susceptibility to antibiotics, which was not influenced by the presence of the efflux inhibitor 'carbonyl cyanide m-chlorophenyl hydrazone'. Outer membranes of adapted strain showed altered protein profile when examined by SDS-PAGE.

译文

在铜绿假单胞菌PAO1 (P.a.) 中确定了对杀菌剂2-苯氧乙醇 (PE) 浓度增加的生长适应性,这是了解和控制杀菌剂耐受性及其对交叉耐药性的影响的一部分。其他杀菌剂和对抗生素的抗性。在含有增加的亚最小抑制性PE浓度的培养基中重复传代培养后,P.a.表现出适应性抗性,在第10代时MIC增加了两倍。抗性稳定,并在无PE的生长培养基中连续7次传代菌株后保持不变。该菌株显示出对异种杀菌剂的交叉抗性,并显示出对抗生素的敏感性增加,这不受外排抑制剂 “羰基氰化物间氯苯基肼” 的影响。当通过sdds-PAGE检查时,适应菌株的外膜显示出改变的蛋白质谱。

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