A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify (1)H/(13)C sugar spin systems in (13)C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of (13)C-(1)H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1'- and 3'-CH signal dispersion for complete spin system identification including 5'-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3'-untranslated region of the pre-mRNA of human U1A protein.

译文

结合 (4,3)D HCCH提出了新实施的G矩阵傅里叶变换 (GFT) (4,3)D HC(C)CH实验,以有效地鉴定 (13)C标记核酸中的 (1)H/(13)C糖自旋系统。该实验能够快速收集高度分辨的中继4D HC(C)CH光谱信息,即由两个碳键分开的 (13)C-(1)H基团的移位相关性。对于RNA,(4,3)D HC(C)CH利用相对有利的1 '-和3'-CH信号分散来进行包括5 '-CH的完整自旋系统鉴定。基于 (4,3) dhc (C)CH/HCCH的策略是针对人U1A蛋白的前mRNA的30-核苷酸3 '-非翻译区的例子。

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