The root of Paeonia lactiflora Pall. (Chishao, CS) and Ligusticum chuanxiong Hort. (Chuanxiong, CX) were widely used as a drug pair in Chinese Medicine, and the combination of CS and CX showed a more significant inhibition on neuronal apoptosis in our previous study. In the present study, total paeony glycosides (TPGs) from CS and total ligustici phenolic acids (TLPAs) from CX were combined to evaluate the synergistic effects against focal cerebral ischemia both in vitro and in vivo. The combination of TPGs and TLPAs at 7:3 had the best anti-oxidative stress and anti-inflammatory effect on OGD-induced HUVEC. Additionally, the infarction area proportion and neuron apoptosis of rats by TPGs:TLPAs (7:3) was significantly lower than their alone in MCAO rats. Moreover, TPGs: TLPAs of 7:3 showed a more significant effect on decreasing the expression of MMP-2 and MMP-9, and increasing the protein expression or mRNA level of TIMP-1 than other combinations. The optimal ratio of TPGs and TLPAs at 7:3 could bring more remarkable protective effects against focal cerebral ischemia in MCAO rats by alleviating oxidative stress, inflammatory and neuronal apoptosis to protect the blood-brain barrier. Overall, the present study provided benefical evidence for clinical application of CS and CX as a "drug pair".

译文

芍药的根。(Chishao,CS) 和川芎。(川芎,CX) 在中药中被广泛用作药物对,在我们先前的研究中,CS和CX的组合对神经元凋亡表现出更明显的抑制作用。在本研究中,将来自CS的总pa苷 (TPGs) 和来自CX的总ligustici酚酸 (TLPAs) 组合在一起,以评估体外和体内对局灶性脑缺血的协同作用。7:3时TPGs和TLPAs的组合对OGD诱导的HUVEC具有最佳的抗氧化应激和抗炎作用。此外,在MCAO大鼠中,TPGs: tlpa (7:3) 对大鼠的梗死面积比例和神经元凋亡显着低于单独使用。此外,与其他组合相比,7:3的TPGs: tlpa在降低MMP-2和MMP-9的表达以及增加TIMP-1的蛋白表达或mRNA水平方面显示出更显着的作用。7:3 TPGs和TLPAs的最佳比例可以通过减轻氧化应激,炎症和神经元凋亡来保护血脑屏障,从而对MCAO大鼠局灶性脑缺血产生更明显的保护作用。总体而言,本研究为CS和CX作为 “药物对” 的临床应用提供了有益的证据。

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