Human parvovirus B19 (B19) IgG was studied retrospectively in 66 allogeneic bone marrow transplantation (BMT) patients using an enzyme-linked immunosorbent assay. Recipient and donor sera had been stored pre-BMT together with sequential sera thereafter. Approximately half of donors and recipients had anti-B19 IgG pre-BMT and thus the relative contributions of donor and recipient immunity to antibody production after transplantation could be assessed. For each patient, a serum taken 2 to 3 years after BMT was also tested and the results show that persistence of B19 antibody depends on prior recipient (P = .0003) but not on donor immunity (P = .8). The findings were similar in both sibling and (VUD) BMT volunteer unrelated donor patients. Analysis of sequential post-BMT sera from 41 of the patients, for whom appropriately timed samples were available, showed primary B19 infection in 3 seronegative individuals, whereas 5 others who were seropositive before BMT underwent recurrent infection. Sequential results from the remaining 33 patients without recent B19 infection showed no evidence for donor antibody transfer and confirmed that antibody persistence depends on prior recipient immunity. B19 IgG levels decreased variably with time and some patients eventually became seronegative. It is concluded that this long-term persistence of B19 antibody post-BMT is most probably due to the existence of long-lived recipient plasma cells.

译文

使用酶联免疫吸附测定法对66例同种异体骨髓移植 (BMT) 患者进行了人细小病毒B19 (B19) IgG的回顾性研究。受体和供体血清已在BMT之前与随后的连续血清一起存储。大约一半的供体和受体具有anti-B19的IgG pre-BMT,因此可以评估供体和受体免疫对移植后抗体产生的相对贡献。对于每个患者,还测试了BMT后2至3年的血清,结果表明B19抗体的持久性取决于先前的接受者 (P = .0003),而不取决于供体免疫 (P = .8)。兄弟姐妹和 (VUD) BMT志愿无关的供体患者的发现相似。对41名患者的BMT后血清的顺序分析 (可提供适当的定时样本) 显示,3名血清阴性个体的原发性B19感染,而其他5名在BMT复发性感染之前血清呈阳性。其余33例近期未感染B19的患者的连续结果显示没有供体抗体转移的证据,并证实抗体的持久性取决于先前的受体免疫力。B19 IgG水平随时间变化而降低,一些患者最终出现血清阴性。结论是,B19抗体在BMT后的这种长期持久性很可能是由于存在长寿的受体浆细胞。

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